exRNAdisease

General Information

Database ID:

exR0000289

RNA Name:

ADCY6

RNA Type:

mRNA

Chromosome:

chr12

Starnd:

Coordinate:

Start Site(bp):

48766194

End Site(bp):

48789037

External Links:

Disease Information

Disease Name:	Down Syndrome
Disease Category:	Congenital, Hereditary, and Neonatal Diseases and Abnormalities
MeSH ID:	D004314
Type:	Diseases Category/Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Alias:	Syndrome, Down//Mongolism//47,XY,+21//Trisomy G//47,XX,+21//Down's Syndrome//Downs Syndrome//Syndrome, Down's//Trisomy 21//Trisomy 21, Mitotic Nondisjunction//Down Syndrome, Partial Trisomy 21//Partial Trisomy 21 Down Syndrome//Trisomy 21, Meiotic Nondisjunction

Expression Detail

GEO ID:	GSE16176
Description:	Expression profiles of amniotic fluid from human fetuses with Down syndrome and euploid controls
Experimental Design:	Disease vs Control
Case Disease Type:	Down Syndrome
Case Disease SubType:	NA
Case Sample:	Down Syndrome
Control Sample:	Normal
Number of Case:	7
Number of Control:	7
Number of Samples:	14

Regulatory Relationship

mRNA targets:

Gene Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
MACF1	chr1	39081316	39487177	+
SLC25A29	chr14	100291116	100306547	-
HSP90AB1	chr6	44246166	44253888	+
CALM1	chr14	90396502	90408268	+
PRRT4	chr7	128350325	128361685	-
LCMT1	chr16	25111731	25178231	+
FLNA	chrX	154348524	154374638	-
TPM4	chr19	16067021	16103002	+
FADS2	chr11	61792980	61867354	+
MYBBP1A	chr17	4538897	4555631	-
UBB	chr17	16380798	16382745	+
RNF167	chr17	4940008	4945222	+
PUS1	chr12	131929200	131945896	+
LRCH4	chr7	100574011	100586129	-
SELENOP	chr5	42799880	42887392	-
ARID1A	chr1	26693236	26782104	+
PEX19	chr1	160276812	160286348	-
IGF2	chr11	2129112	2141238	-
FNDC3B	chr3	172039578	172401669	+
HUWE1	chrX	53532096	53686728	-
PDCD7	chr15	65117379	65133808	-
LENG8	chr19	54448887	54462037	+
SYNM	chr15	99098217	99135593	+
EME2	chr16	1772810	1781708	+
FNTB	chr14	64986895	65062652	+
SMARCD1	chr12	50085200	50100707	+
SNTB2	chr16	69187129	69309052	+
ZNF628	chr19	55476617	55484487	+
SNRPF	chr12	95858928	95903828	+
TAZ	chrX	154411524	154421726	+

miRNA targets:

circRNA targets:

lncRNA targets:

lncRNA Symbol	Chromosome	Start Site(bp)	End Site(bp)	Strand
AC069281.2	chr7	100572232	100578700	-
AC138761.1	chr17	22266395	22288301	+
XIST	chrX	73820649	73852723	-

Display:

Experiment Detail

GEO ID:	GSE16176
Sample Source:	Amniotic Fluid
Source Fraction:	Supernatant
Platform:	GPL570
Method:	Microarray
Num of detected RNA Type:	1
Num of detected RNAs of this Type:	17063
Sample treatment protocol:	NA
RNA Extract protocol:	RNA was extracted from 10 mL AF with 30 mL TRIzol LS Reagent (Invitrogen, Carlsbad, CA) and 8 mL chloroform. After RNA extraction, RNA was purified and DNA was removed using the RNeasy® Maxi Kit, including the DNase step according to the manufacturer's protocol (QIAGEN, Valencia, CA). RNA was precipitated using 3M NaOAc and 100% ethanol, and 80% ethanol was added after 4 hours incubation at -20 °C. cDNA, synthesized from extracted RNA, was amplified and purified using the WT-Ovation™ Pico RNA Amplification System (NuGEN, San Carlos, CA) and the DNA Clean & Concentrator™-25 (Zymo Research, Orange, CA).
RNA library preparation protocol:	biotin Samples were labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN, San Carlos, CA).

Reference

PMID:	19474297
Title:	Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
Author:	Slonim DK, Koide K, Johnson KL, Tantravahi U, Cowan JM, Jarrah Z, Bianchi DW
Journal:	Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9425-9.
Description:	To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples.