| GEO ID: | GSE106224 |
| Sample Source: | Blood |
| Source Fraction: | Plasma |
| Platform: | GPL18573 |
| Method: | NGS |
| Num of detected RNA Type: | 1 |
| Num of detected RNAs of this Type: | 1522 |
| Sample treatment protocol: | Whole blood samples were centrifuged for 15 minutes at 2,000×g at 4°C to get plasma. EVs were isolated from 100 ul of plasma using size exclusion columns (iZON qEV, Cambridge MA) with de-gassed 1X PBS from a total of 22 selected samples, 11 from each group (Table 1). Eluate (~500 ul for each fraction) was collected in microfuge tubes individually. The protein contains and concentration for each fraction were assessed by protein gel electrophoresis and Bradford assay (BioRad, Hercules, CA). |
| RNA Extract protocol: | RNA was isolated from all plasma samples, the 22 EV samples, and 22 corresponding EV-depleted fractions using miRNeasy Micro Kit (Qiagne, Germantown MD). |
| RNA library preparation protocol: | Small RNA sequencing libraries were generated with a modified small RNAseq protocol (manuscript in preparation) that alleviates most of the adapter-miRNA ligation sequence bias problem. |