Entry Detail



General Information

Database ID:exR0226366
RNA Name:hsa-let-7f-5p
RNA Type:miRNA
Chromosome:chrX
Starnd:-
Coordinate:
Start Site(bp):53557246End Site(bp):53557267
External Links:hsa-let-7f-5p



Disease Information

Disease Name:Active Tuberculosis
Disease Category:Infections
MeSH ID:D014376
Type:Diseases Category/Infections
Alias:Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections



Expression Detail

GEO ID:GSE124120
Description:Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis
Experimental Design:Disease vs Control
Case Disease Type:Active Tuberculosis
Case Disease SubType:NA
Case Sample:Active Tuberculosis
Control Sample:Healthy
Number of Case:1
Number of Control:1
Number of Samples:2





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ACP1
chr2
264140
278283
+
ARHGAP28
chr18
6729718
6915716
+
BLOC1S6
chr15
45587214
45615945
+
CTPS2
chrX
16587999
16712936
-
EEA1
chr12
92770637
92929331
-
GRPEL2
chr5
149345430
149354583
+
HDLBP
chr2
241227264
241317061
-
HMGA2
chr12
65824131
65966295
+
IGF2BP1
chr17
48997385
49056145
+
NHLRC3
chr13
39038306
39050109
+
PDPR
chr16
70113626
70162537
+
PHC3
chr3
170086732
170181749
-
RANBP2
chr2
108719482
108785809
+
RNH1
chr11
494512
507300
-
TBC1D13
chr9
128787253
128810430
+
TRIM65
chr17
75880335
75896951
-
UBE2G2
chr21
44768580
44801826
-
ACTG1
chr17
81509971
81523847
-
C18orf21
chr18
35972625
35979286
+
FAM162A
chr3
122384176
122412334
+
GSTO1
chr10
104235356
104267459
+
HLA-DQA2
chr6
32741391
32747198
+
LONP1
chr19
5691834
5720572
-
PFKFB4
chr3
48517684
48562015
-
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000247
chr10
74474868
74475660
+
hsa_circ_0000987
chr2
30748452
30756180
+
hsa_circ_0000246
chr10
74468040
74475660
+
hsa_circ_0000018
chr1
15860731
15863309
+
hsa_circ_0000038
chr1
28800065
28802803
+
hsa_circ_0000799
chr17
65941524
65972074
+
hsa_circ_0001164
chr20
45891031
45923523
-
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC006064.5
chr12
6510275
6510522
+
AC074117.1
chr2
27356246
27367622
+
AC109460.3
chr16
28974804
28990775
+
AC124045.1
chr3
44667412
44669364
+
AP000766.1
chr11
107312132
107316271
-
ARHGAP27P1-BPTFP1-KPNA2P3
chr17
64749663
64781707
-
KCNQ1OT1
chr11
2608328
2699994
-
LINC00265
chr7
39733430
39793092
+
LINC02381
chr12
54126082
54147485
+
MIRLET7BHG
chr22
46053869
46113928
+
NEAT1
chr11
65422774
65445540
+
NUTM2A-AS1
chr10
87201647
87342612
-
SNHG16
chr17
76557764
76565348
+
SNHG4
chr5
139274102
139284899
+
TMPO-AS1
chr12
98512973
98516422
-
XIST
chrX
73820649
73852723
-
ZNF436-AS1
chr1
23368997
23371839
+
Display:



Experiment Detail

GEO ID:GSE124120
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL16791
Method:NGS
Num of detected RNA Type:1
Num of detected RNAs of this Type:356
Sample treatment protocol:We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions.
RNA Extract protocol:NA
RNA library preparation protocol:Small RNA libraries were prepared for sequencing using standard Illumina protocols.



Reference

PMID:https://doi.org/10.3389/fmicb.2019.01174
Title:Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
Author:Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang
Journal:Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174
Description:In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).