Entry Detail



General Information

Database ID:exR0226373
RNA Name:hsa-miR-10b-5p
RNA Type:miRNA
Chromosome:chr2
Starnd:+
Coordinate:
Start Site(bp):176150329End Site(bp):176150351
External Links:hsa-miR-10b-5p



Disease Information

Disease Name:Active Tuberculosis
Disease Category:Infections
MeSH ID:D014376
Type:Diseases Category/Infections
Alias:Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections



Expression Detail

GEO ID:GSE124120
Description:Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis
Experimental Design:Disease vs Control
Case Disease Type:Active Tuberculosis
Case Disease SubType:NA
Case Sample:Active Tuberculosis
Control Sample:Healthy
Number of Case:1
Number of Control:1
Number of Samples:2





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ARPC5
chr1
183620846
183635783
-
RAP2A
chr13
97434169
97469128
+
SCAMP5
chr15
74957219
75021495
+
miRNA targets:NA
circRNA targets:NA
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC004069.1
chr4
105137280
105140619
-
GAS5
chr1
173858559
173868882
-
LINC00963
chr9
129476946
129513687
+
LINC01578
chr15
92819540
92899701
+
NEAT1
chr11
65422774
65445540
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE124120
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL16791
Method:NGS
Num of detected RNA Type:1
Num of detected RNAs of this Type:356
Sample treatment protocol:We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions.
RNA Extract protocol:NA
RNA library preparation protocol:Small RNA libraries were prepared for sequencing using standard Illumina protocols.



Reference

PMID:https://doi.org/10.3389/fmicb.2019.01174
Title:Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
Author:Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang
Journal:Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174
Description:In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).