Entry Detail


General Information

Database ID:exR0226383
RNA Name:hsa-miR-134-5p
RNA Type:miRNA
Chromosome:chr14
Starnd:+
Coordinate:
Start Site(bp):101054694End Site(bp):101054715
External Links:hsa-miR-134-5p



Disease Information

Disease Name:Active Tuberculosis
Disease Category:Infections
MeSH ID:D014376
Type:Diseases Category/Infections
Alias:Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections



Expression Detail

GEO ID:GSE124120
Description:Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis
Experimental Design:Disease vs Control
Case Disease Type:Active Tuberculosis
Case Disease SubType:NA
Case Sample:Active Tuberculosis
Control Sample:Healthy
Number of Case:1
Number of Control:1
Number of Samples:2





Regulatory Relationship

mRNA targets:NA
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000642
chr15
80390757
80415142
+
hsa_circ_0000643
chr15
80412669
80415142
+
hsa_circ_0000798
chr17
65941524
65944422
+
hsa_circ_0000799
chr17
65941524
65972074
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC004687.1
chr17
58330884
58332508
-
AC009948.3
chr2
178541125
178541799
+
AC092127.1
chr16
85592266
85595720
+
AC097634.1
chr3
71584943
71587409
+
AC144548.1
chr12
110387463
110445548
-
AC145207.5
chr17
81878425
81881106
-
ADAMTSL4-AS1
chr1
150560202
150574552
-
DNM3OS
chr1
172138397
172144840
-
ENTPD3-AS1
chr3
40313802
40453329
-
KCNQ1OT1
chr11
2608328
2699994
-
LINC01278
chrX
63222993
63561095
-
NEAT1
chr11
65422774
65445540
+
PINK1-AS
chr1
20642657
20652193
-
TDRKH-AS1
chr1
151790804
151794402
+
Display:



Experiment Detail

GEO ID:GSE124120
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL16791
Method:NGS
Num of detected RNA Type:1
Num of detected RNAs of this Type:356
Sample treatment protocol:We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions.
RNA Extract protocol:NA
RNA library preparation protocol:Small RNA libraries were prepared for sequencing using standard Illumina protocols.



Reference

PMID:https://doi.org/10.3389/fmicb.2019.01174
Title:Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
Author:Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang
Journal:Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174
Description:In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).