exRNAdisease

Entry Detail

---



General Information

Database ID: exR0226391 RNA Name: hsa-miR-16-5p RNA Type: miRNA Chromosome: chr3 Starnd: + Coordinate: Start Site(bp): 160404754 End Site(bp): 160404775 External Links: hsa-miR-16-5p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AMER1 chrX 64185117 64205708 - ANKRD17 chr4 73073376 73258798 - ARHGAP35 chr19 46860997 47005077 + ARHGDIA chr17 81867721 81871378 - ARRDC4 chr15 97960703 97973833 + ASB1 chr2 238426742 238452250 + ATXN7L3 chr17 44191805 44200113 - BACE1 chr11 117285207 117316259 - BIRC5 chr17 78214186 78225636 + CYB561A3 chr11 61348745 61362299 - DCAF7 chr17 63550477 63594279 + FAM89A chr1 231018958 231040254 - FLOT2 chr17 28879335 28897733 - FSD1 chr19 4304598 4323843 + ITPR1 chr3 4493345 4847840 + JARID2 chr6 15246069 15522042 + KDSR chr18 63327726 63367228 - MAPK1IP1L chr14 55051647 55070194 + MAPKBP1 chr15 41774434 41827855 + PLPP6 chr9 4662294 4665258 + RTL8B chrX 135020513 135022542 - SEC61A1 chr3 128051641 128071683 + STEAP3 chr2 119223831 119265652 + TBRG1 chr11 124622836 124635926 + TOB2 chr22 41433494 41446801 - UNC119B chr12 120710458 120723640 + ZBTB34 chr9 126860665 126885878 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000540 chr14 55799534 55821940 + hsa_circ_0001005 chr2 53921020 53978078 - hsa_circ_0001723 chr7 91924202 91948826 + hsa_circ_0000458 chr12 124071293 124074996 + hsa_circ_0001699 chr7 40027197 40041630 + hsa_circ_0001387 chr4 1902352 1936989 + hsa_circ_0001168 chr20 47691321 47707559 + hsa_circ_0000552 chr14 71880664 71948928 + hsa_circ_0000038 chr1 28800065 28802803 + hsa_circ_0000652 chr15 90984737 90986710 + hsa_circ_0001472 chr5 36953719 36976504 + hsa_circ_0001263 chr3 5215701 5216099 + hsa_circ_0000799 chr17 65941524 65972074 + hsa_circ_0001164 chr20 45891031 45923523 - hsa_circ_0000032 chr1 25666964 25669564 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC079781.5 chr7 97851688 97972985 - HCG18 chr6 30286690 30327382 - XIST chrX 73820649 73852723 - FGD5-AS1 chr3 14920347 14948424 - LINC00649 chr21 33915534 33977691 + AC009022.1 chr16 69976297 70065948 - AC114980.1 chr5 93860669 93863825 - AC002470.2 chr22 20979462 20998121 + SNHG16 chr17 76557764 76565348 + LINC01184 chr5 127939152 128083172 - NEAT1 chr11 65422774 65445540 + AC021078.1 chr5 149494314 149504670 - AL359924.1 chr1 237862175 237928321 + LINC02035 chr3 122886941 122892416 + NUTM2B-AS1 chr10 79661394 79826594 - SNHG25 chr17 64145970 64146476 + AC003092.1 chr7 94022833 94066661 + AP000317.2 chr21 34073592 34360033 + MIR497HG chr17 7015818 7019659 - SNHG12 chr1 28578538 28583132 - MIR29B2CHG chr1 207801518 207879115 - AC020978.7 chr16 68224713 68227734 + MZF1-AS1 chr19 58559129 58574797 + SNHG1 chr11 62851984 62855953 - MCM3AP-AS1 chr21 46229196 46259390 + LINC00662 chr19 27681072 27794005 - NUTM2A-AS1 chr10 87201647 87342612 - AL031282.2 chr1 1702736 1737688 - MIR503HG chrX 134543119 134546642 - AL391244.1 chr1 1420245 1422691 + Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).