Entry Detail



General Information

Database ID:exR0226395
RNA Name:hsa-miR-186-5p
RNA Type:miRNA
Chromosome:chr1
Starnd:-
Coordinate:
Start Site(bp):71067681End Site(bp):71067702
External Links:hsa-miR-186-5p



Disease Information

Disease Name:Active Tuberculosis
Disease Category:Infections
MeSH ID:D014376
Type:Diseases Category/Infections
Alias:Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections



Expression Detail

GEO ID:GSE124120
Description:Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis
Experimental Design:Disease vs Control
Case Disease Type:Active Tuberculosis
Case Disease SubType:NA
Case Sample:Active Tuberculosis
Control Sample:Healthy
Number of Case:1
Number of Control:1
Number of Samples:2





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
DDX3X
chrX
41333284
41364472
+
EP300
chr22
41092592
41180077
+
GPRC5A
chr12
12890782
12917937
+
NRF1
chr7
129611720
129757082
+
TACSTD2
chr1
58575433
58577252
-
TMEM245
chr9
109015135
109119947
-
TNFRSF21
chr6
47231532
47309905
-
TNKS1BP1
chr11
57299638
57324952
-
HMG20A
chr15
77420412
77485607
+
RNF139
chr8
124474738
124487914
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0000892
chr19
11941431
12014514
+
hsa_circ_0000799
chr17
65941524
65972074
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC021078.1
chr5
149494314
149504670
-
AC097634.1
chr3
71584943
71587409
+
AC124798.1
chr11
17380649
17383531
+
AC247036.1
chr14
106206603
106208571
+
DLEU2
chr13
49956670
50125720
-
DSCAM-AS1
chr21
40383083
40385358
+
EBLN3P
chr9
37079645
37090928
+
NEAT1
chr11
65422774
65445540
+
OIP5-AS1
chr15
41283990
41309737
+
SNHG3
chr1
28505980
28510892
+
SNHG7
chr9
136721366
136728184
-
TUG1
chr22
30969245
30979395
+
XIST
chrX
73820649
73852723
-
Display:



Experiment Detail

GEO ID:GSE124120
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL16791
Method:NGS
Num of detected RNA Type:1
Num of detected RNAs of this Type:356
Sample treatment protocol:We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions.
RNA Extract protocol:NA
RNA library preparation protocol:Small RNA libraries were prepared for sequencing using standard Illumina protocols.



Reference

PMID:https://doi.org/10.3389/fmicb.2019.01174
Title:Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
Author:Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang
Journal:Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174
Description:In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).