exRNAdisease

Entry Detail

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General Information

Database ID: exR0226403 RNA Name: hsa-miR-20a-5p RNA Type: miRNA Chromosome: chr13 Starnd: + Coordinate: Start Site(bp): 91351072 End Site(bp): 91351094 External Links: hsa-miR-20a-5p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand RIPOR1 chr16 67518418 67546788 + EIF5A2 chr3 170888418 170908644 - RCCD1 chr15 90954870 90963125 + FHL1 chrX 136146702 136211359 + ZFAND4 chr10 45615500 45672780 - CLOCK chr4 55427903 55546909 - NSD1 chr5 177133025 177300215 + ZDHHC12 chr9 128720870 128724127 - PXYLP1 chr3 141228726 141367753 + C9orf40 chr9 74946583 74952912 - PBXIP1 chr1 154944076 154956123 - ZNF468 chr19 52838010 52857649 - ATXN1 chr6 16299112 16761491 - SCAMP2 chr15 74843730 74873365 - SLC46A3 chr13 28700064 28718970 - GNAS chr20 58839718 58911192 + MBP chr18 76978827 77133683 - AMPD2 chr1 109616104 109632053 + PXDN chr2 1631887 1744852 - WDR37 chr10 1049538 1132384 + CYLD chr16 50742050 50801935 + ZSCAN26 chr6 28267010 28278224 + STRN3 chr14 30893799 31026401 - ADARB1 chr21 45073853 45226560 + KLHL28 chr14 44924319 45042322 - WASF3 chr13 26557683 26688948 + BTN3A1 chr6 26402237 26415208 + CRCP chr7 66114604 66154568 + FCF1 chr14 74713144 74738620 + ARMC8 chr3 138187248 138298384 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001495 chr5 68470703 68471364 + hsa_circ_0000494 chr13 76134888 76143643 + hsa_circ_0000018 chr1 15860731 15863309 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC015871.3 chr15 79920195 79922455 - AC021078.1 chr5 149494314 149504670 - AC021092.1 chr19 44103007 44113183 - AC024940.6 chr12 31280422 31280895 - AC084082.1 chr8 66112667 66126632 + AC087477.2 chr15 96354237 96405235 + AC243964.3 chr19 44631573 44725217 - AL031428.1 chr1 23020147 23088058 - AL137782.1 chr13 75549773 75807120 + AL158206.1 chr9 19453209 19455173 + AL161772.1 chr13 20699307 20703718 - ARHGAP27P1-BPTFP1-KPNA2P3 chr17 64749663 64781707 - CKMT2-AS1 chr5 81201341 81301565 - EPB41L4A-AS1 chr5 112160526 112164818 + ERICD chr8 140636281 140638283 + FGD5-AS1 chr3 14920347 14948424 - H19 chr11 1995176 2001470 - HAGLR chr2 176164051 176188958 - HOTAIR chr12 53962308 53974956 - MALAT1 chr11 65497688 65506516 + NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - SNHG16 chr17 76557764 76565348 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).