exRNAdisease

Entry Detail

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General Information

Database ID: exR0226423 RNA Name: hsa-miR-320d RNA Type: miRNA Chromosome: chr13 Starnd: - Coordinate: Start Site(bp): 40727828 End Site(bp): 40727846 External Links: hsa-miR-320d

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACAP2 chr3 195274745 195443044 - ASB1 chr2 238426742 238452250 + BLCAP chr20 37492472 37527931 - CGN chr1 151510510 151538692 + FJX1 chr11 35618460 35620865 + GMCL1 chr2 69829660 69881384 + MIER3 chr5 56919602 56971675 - PFN1 chr17 4945652 4949061 - SIN3A chr15 75369379 75455842 - TRIM14 chr9 98069275 98119222 - ZCCHC2 chr18 62523025 62587709 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000417 chr12 69644908 69656342 + hsa_circ_0000540 chr14 55799534 55821940 + hsa_circ_0000095 chr1 95609446 95616975 + hsa_circ_0000744 chr17 15968183 15971435 - hsa_circ_0000652 chr15 90984737 90986710 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC002044.1 chr16 73014319 73014476 - AC016876.2 chr17 7581964 7584086 - AC092718.4 chr16 81030770 81031485 + AL022311.1 chr22 37876148 37895563 + AL162258.1 chr1 153626332 153634340 - DLEU1 chr13 50082169 50906856 + GAS5 chr1 173858559 173868882 - LINC00641 chr14 21200079 21206900 - MALAT1 chr11 65497688 65506516 + MIR3142HG chr5 160438594 160487426 + NEAT1 chr11 65422774 65445540 + NR2F2-AS1 chr15 96110040 96327361 - OIP5-AS1 chr15 41283990 41309737 + SNHG1 chr11 62851984 62855953 - SNHG12 chr1 28578538 28583132 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).