exRNAdisease

Entry Detail

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General Information

Database ID: exR0226424 RNA Name: hsa-miR-340-5p RNA Type: miRNA Chromosome: chr5 Starnd: - Coordinate: Start Site(bp): 180015361 End Site(bp): 180015382 External Links: hsa-miR-340-5p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand FAM160B1 chr10 114821744 114899832 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001731 chr7 100731990 100738448 + hsa_circ_0000118 chr1 117944807 117963271 + hsa_circ_0000598 chr15 45009906 45009989 + hsa_circ_0000120 chr1 117944807 118009049 + hsa_circ_0000119 chr1 117944807 117984947 + hsa_circ_0000117 chr1 117944807 117957453 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC020978.7 chr16 68224713 68227734 + AC093010.3 chr3 114314501 114329714 - AC145207.5 chr17 81878425 81881106 - AL158206.1 chr9 19453209 19455173 + EBLN3P chr9 37079645 37090928 + ERICD chr8 140636281 140638283 + LINC00662 chr19 27681072 27794005 - MCM3AP-AS1 chr21 46229196 46259390 + NEAT1 chr11 65422774 65445540 + OIP5-AS1 chr15 41283990 41309737 + TUG1 chr22 30969245 30979395 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).