exRNAdisease

Entry Detail

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General Information

Database ID: exR0226441 RNA Name: hsa-miR-532-5p RNA Type: miRNA Chromosome: chrX Starnd: + Coordinate: Start Site(bp): 50003167 End Site(bp): 50003188 External Links: hsa-miR-532-5p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AXL chr19 41219223 41261766 + CRIM1 chr2 36355778 36551135 + DCTN4 chr5 150708440 150759109 - LEPROTL1 chr8 30095408 30177208 + LUZP1 chr1 23084023 23177808 - MEX3A chr1 156072013 156082465 - PRRC1 chr5 127517609 127555089 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000787 chr17 54978779 54981849 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC079781.5 chr7 97851688 97972985 - AL049796.1 chr1 93847174 93848939 + AP000553.1 chr22 21652270 21670237 + GAS5 chr1 173858559 173868882 - KCNQ1OT1 chr11 2608328 2699994 - RRN3P2 chr16 29074976 29116718 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).