exRNAdisease

Entry Detail

---



General Information

Database ID: exR0226445 RNA Name: hsa-miR-671-5p RNA Type: miRNA Chromosome: chr7 Starnd: + Coordinate: Start Site(bp): 151238449 End Site(bp): 151238471 External Links: hsa-miR-671-5p

Disease Information

Disease Name: Active Tuberculosis Disease Category: Infections MeSH ID: D014376 Type: Diseases Category/Infections Alias: Tuberculosis//Tuberculoses//Kochs Disease//Koch's Disease//Koch Disease//Mycobacterium tuberculosis Infection//Infection, Mycobacterium tuberculosis//Infections, Mycobacterium tuberculosis//Mycobacterium tuberculosis Infections

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Active Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand RCOR1 chr14 102592649 102730561 + KIAA0232 chr4 6781375 6884170 + TAF8 chr6 42050513 42087461 + MAFG chr17 81918270 81927735 - DYRK1A chr21 37365573 37526358 + MED22 chr9 133338312 133348131 - KLC1 chr14 103561896 103714249 + CBX6 chr22 38861422 38872249 - SAMD4B chr19 39342396 39385710 + GTSE1 chr22 46296870 46330810 + SETD5 chr3 9397615 9479240 + CREG1 chr1 167529117 167553805 - IRS2 chr13 109752695 109786583 - SCAMP3 chr1 155255979 155262430 - CCDC6 chr10 59788747 59906556 - BAZ2A chr12 56595596 56636816 - WDR82 chr3 52254434 52288020 - TOMM6 chr6 41787662 41789898 + FAM219A chr9 34398184 34458570 - STAT3 chr17 42313324 42388568 - UHRF1BP1 chr6 34792015 34883138 + AL365205.1 chr6 41780349 41790141 + NACC1 chr19 13116862 13141147 + SMAD3 chr15 67063763 67195169 + WDTC1 chr1 27234632 27308636 + IL1R1 chr2 102064544 102179874 + PTPRU chr1 29236516 29326813 + GLYR1 chr16 4803203 4847288 - APLP2 chr11 130068147 130144811 + CHTF8 chr16 69118010 69132578 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC067930.1 chr8 143579636 143580670 + AC145207.5 chr17 81878425 81881106 - AL022311.1 chr22 37876148 37895563 + AL137127.1 chr1 19072110 19075511 - H19 chr11 1995176 2001470 - KCNQ1OT1 chr11 2608328 2699994 - LINC01278 chrX 63222993 63561095 - MIR29B2CHG chr1 207801518 207879115 - NEAT1 chr11 65422774 65445540 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).