Entry Detail



General Information

Database ID:exR0226470
RNA Name:hsa-miR-143-3p
RNA Type:miRNA
Chromosome:chr5
Starnd:+
Coordinate:
Start Site(bp):149428978End Site(bp):149428998
External Links:hsa-miR-143-3p



Disease Information

Disease Name:Latent Tuberculosis
Disease Category:Infections
MeSH ID:D055985
Type:Diseases Category/Infections
Alias:Latent Tuberculoses//Tuberculoses, Latent//Tuberculosis, Latent//Latent Tuberculosis Infection//Infection, Latent Tuberculosis//Infections, Latent Tuberculosis//Latent Tuberculosis Infections//Tuberculosis Infection, Latent//Tuberculosis Infections, Latent



Expression Detail

GEO ID:GSE124120
Description:Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis
Experimental Design:Disease vs Control
Case Disease Type:Active Tuberculosis
Case Disease SubType:NA
Case Sample:Latent Tuberculosis
Control Sample:Healthy
Number of Case:1
Number of Control:1
Number of Samples:2





Regulatory Relationship

mRNA targets:
Gene SymbolChromosomeStart Site(bp)End Site(bp)Strand
ACVR1B
chr12
51951699
51997078
+
BTF3L4
chr1
52056199
52090716
+
BTG2
chr1
203305491
203309602
+
CAPZB
chr1
19338775
19485539
-
CDK6
chr7
92604921
92836594
-
CELSR2
chr1
109249539
109275751
+
CIT
chr12
119685791
119877320
-
DEGS1
chr1
224175756
224193441
+
DYRK1B
chr19
39825350
39834201
-
EFNA3
chr1
155078837
155087538
+
FOSL2
chr2
28392448
28417317
+
IGFBP5
chr2
216672105
216695549
-
KRIT1
chr7
92198969
92246166
-
MAP3K3
chr17
63622415
63696303
+
NES
chr1
156668763
156677407
-
PACS1
chr11
66070272
66244744
+
PATL1
chr11
59636716
59669037
-
PNKD
chr2
218270392
218346793
+
RFWD3
chr16
74621399
74666877
-
SLC7A11
chr4
138164097
138242349
-
SON
chr21
33543038
33577481
+
STARD4
chr5
111496033
111512590
-
STMN3
chr20
63639705
63657682
-
TATDN2
chr3
10248023
10281218
+
TFPI
chr2
187464230
187565760
-
TIGD5
chr8
143597831
143603224
+
VPS37C
chr11
61130257
61161615
-
WSB1
chr17
27294076
27315926
+
miRNA targets:NA
circRNA targets:
circRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
hsa_circ_0001247
chr22
46125304
46136418
+
hsa_circ_0000711
chr16
68155889
68160513
+
hsa_circ_0000431
chr12
102301963
102308010
+
hsa_circ_0001168
chr20
47691321
47707559
+
lncRNA targets:
lncRNA SymbolChromosomeStart Site(bp)End Site(bp)Strand
AC009022.1
chr16
69976297
70065948
-
AC091564.7
chr11
6610883
6616594
-
AC245884.8
chr19
54438665
54439544
-
DHRS4-AS1
chr14
23938219
24052555
-
LINC00667
chr18
5237826
5290608
+
LZTS1-AS1
chr8
20275773
20290458
+
MALAT1
chr11
65497688
65506516
+
MCM3AP-AS1
chr21
46229196
46259390
+
OIP5-AS1
chr15
41283990
41309737
+
Display:



Experiment Detail

GEO ID:GSE124120
Sample Source:Blood
Source Fraction:Exosome
Platform:GPL16791
Method:NGS
Num of detected RNA Type:1
Num of detected RNAs of this Type:356
Sample treatment protocol:We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions.
RNA Extract protocol:NA
RNA library preparation protocol:Small RNA libraries were prepared for sequencing using standard Illumina protocols.



Reference

PMID:https://doi.org/10.3389/fmicb.2019.01174
Title:Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
Author:Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang
Journal:Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174
Description:In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).