exRNAdisease

Entry Detail

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General Information

Database ID: exR0226471 RNA Name: hsa-miR-145-5p RNA Type: miRNA Chromosome: chr5 Starnd: + Coordinate: Start Site(bp): 149430661 End Site(bp): 149430683 External Links: hsa-miR-145-5p

Disease Information

Disease Name: Latent Tuberculosis Disease Category: Infections MeSH ID: D055985 Type: Diseases Category/Infections Alias: Latent Tuberculoses//Tuberculoses, Latent//Tuberculosis, Latent//Latent Tuberculosis Infection//Infection, Latent Tuberculosis//Infections, Latent Tuberculosis//Latent Tuberculosis Infections//Tuberculosis Infection, Latent//Tuberculosis Infections, Latent

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Latent Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACBD5 chr10 27195214 27242130 - ATP9A chr20 51596514 51768390 - BAZ1B chr7 73440406 73522293 - CARMIL1 chr6 25279078 25620530 + CCNJ chr10 96043394 96060870 + MYRF chr11 61752642 61788518 + PLEKHM1 chr17 45435900 45490749 - PPP1R9B chr17 50133737 50150677 - RAD23B chr9 107283137 107332192 + ZNF28 chr19 52797408 52857600 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001454 chr4 154524454 154533552 + hsa_circ_0000540 chr14 55799534 55821940 + hsa_circ_0001731 chr7 100731990 100738448 + hsa_circ_0001492 chr5 65284462 65290692 + hsa_circ_0001414 chr4 56277780 56284152 + hsa_circ_0000943 chr19 47421744 47440665 + hsa_circ_0001168 chr20 47691321 47707559 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC006064.5 chr12 6510275 6510522 + AC016876.2 chr17 7581964 7584086 - AC023509.1 chr12 53441741 53467528 + AC105339.2 chr15 82752884 82757208 + AC144548.1 chr12 110387463 110445548 - AL031282.2 chr1 1702736 1737688 - JPX chrX 73944182 74070408 + KCNQ1OT1 chr11 2608328 2699994 - LINC00852 chr3 10284419 10285746 + MALAT1 chr11 65497688 65506516 + MEG3 chr14 100779410 100861031 + SNHG1 chr11 62851984 62855953 - TUG1 chr22 30969245 30979395 + Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).