exRNAdisease

Entry Detail

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General Information

Database ID: exR0226492 RNA Name: hsa-miR-22-3p RNA Type: miRNA Chromosome: chr17 Starnd: - Coordinate: Start Site(bp): 1713914 End Site(bp): 1713935 External Links: hsa-miR-22-3p

Disease Information

Disease Name: Latent Tuberculosis Disease Category: Infections MeSH ID: D055985 Type: Diseases Category/Infections Alias: Latent Tuberculoses//Tuberculoses, Latent//Tuberculosis, Latent//Latent Tuberculosis Infection//Infection, Latent Tuberculosis//Infections, Latent Tuberculosis//Latent Tuberculosis Infections//Tuberculosis Infection, Latent//Tuberculosis Infections, Latent

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Latent Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand CCDC97 chr19 41310172 41324873 + AP1S1 chr7 101154456 101161596 + RCOR1 chr14 102592649 102730561 + RAB11FIP4 chr17 31391675 31538211 + PPIF chr10 79347469 79355334 + FBXL19 chr16 30923055 30948783 + FNBP4 chr11 47716494 47767443 - DCP1A chr3 53283429 53347586 - GDAP1 chr8 74321130 74488872 + WSB2 chr12 118032694 118062430 - ITGA5 chr12 54395261 54419266 - IRS2 chr13 109752695 109786583 - FOXP1 chr3 70952817 71583993 - TMEM179B chr11 62787402 62790400 + ETS1 chr11 128458761 128587558 - ATP2C1 chr3 130850595 131016712 + ZNF740 chr12 53180704 53195142 + CACUL1 chr10 118674167 118755249 - ATP6V0B chr1 43974487 43978295 + ACLY chr17 41866917 41930542 - CLASP1 chr2 121337776 121649587 - PRLR chr5 35048756 35230487 - PDPR chr16 70113626 70162537 + WWC2 chr4 183099257 183320777 + TAGLN chr11 117199370 117207464 + BCL9 chr1 147541501 147626216 + GLIS2 chr16 4314761 4339597 + GBF1 chr10 102245532 102382899 + PDSS1 chr10 26697701 26746798 + RANBP2 chr2 108719482 108785809 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001699 chr7 40027197 40041630 + hsa_circ_0001414 chr4 56277780 56284152 + hsa_circ_0000733 chr17 1561546 1562030 - lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC021078.1 chr5 149494314 149504670 - AL360012.1 chr1 28648600 28648730 + MALAT1 chr11 65497688 65506516 + NEAT1 chr11 65422774 65445540 + NORAD chr20 36045618 36051018 - OIP5-AS1 chr15 41283990 41309737 + SNHG16 chr17 76557764 76565348 + Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).