exRNAdisease

Entry Detail

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General Information

Database ID: exR0226511 RNA Name: hsa-miR-342-3p RNA Type: miRNA Chromosome: chr14 Starnd: + Coordinate: Start Site(bp): 100109715 End Site(bp): 100109737 External Links: hsa-miR-342-3p

Disease Information

Disease Name: Latent Tuberculosis Disease Category: Infections MeSH ID: D055985 Type: Diseases Category/Infections Alias: Latent Tuberculoses//Tuberculoses, Latent//Tuberculosis, Latent//Latent Tuberculosis Infection//Infection, Latent Tuberculosis//Infections, Latent Tuberculosis//Latent Tuberculosis Infections//Tuberculosis Infection, Latent//Tuberculosis Infections, Latent

Expression Detail

GEO ID: GSE124120 Description: Small RNA Profiles of Serum Exosomes Derived from Individuals with Latent and Active Tuberculosis Experimental Design: Disease vs Control Case Disease Type: Active Tuberculosis Case Disease SubType: NA Case Sample: Latent Tuberculosis Control Sample: Healthy Number of Case: 1 Number of Control: 1 Number of Samples: 2

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AC009133.6 chr16 29812261 29820092 + ACVR1B chr12 51951699 51997078 + CRIM1 chr2 36355778 36551135 + E2F1 chr20 33675477 33686385 - EXOSC6 chr16 70246778 70251940 - IGFBP4 chr17 40443450 40457725 + LASP1 chr17 38869859 38921770 + PAGR1 chr16 29816152 29822489 + UBTF chr17 44205033 44221626 - USP31 chr16 23061406 23149270 - WDR77 chr1 111439890 111449256 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC010327.5 chr19 55216660 55221616 + AC016876.2 chr17 7581964 7584086 - AC021078.1 chr5 149494314 149504670 - AL356488.2 chr1 109100193 109100619 + CKMT2-AS1 chr5 81201341 81301565 - H19 chr11 1995176 2001470 - LINC00894 chrX 149938613 150224580 + NEAT1 chr11 65422774 65445540 + OIP5-AS1 chr15 41283990 41309737 + SNHG20 chr17 77086716 77099902 + SNHG7 chr9 136721366 136728184 - STAG3L5P-PVRIG2P-PILRB chr7 100336104 100367831 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE124120 Sample Source: Blood Source Fraction: Exosome Platform: GPL16791 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 356 Sample treatment protocol: We first performed differential centrifugation (1000 g for 10 min at 4 °C, and 16,500 g for 30 min at 4 °C) to remove the cell debris, followed by ultrafiltration using 0.22 um filters. We then performed ultracentrifugation (120,000 g for 2 hours) to obtain exosome pellets. Transmission electron microscopy (TEM) was employed to visualize exosomes. The exosome pellets were first suspended and dropped onto 200 mesh copper grids, followed by incubation in 3% phosphotungstic acid for 10 min. The copper mesh was then washed three times with water and dried for observation by TEM. We also analyzed the exosome size distributions using the nanoparticle tracking analysis (NTA, Malvern, UK) according to the manufacturer's instructions. RNA Extract protocol: NA RNA library preparation protocol: Small RNA libraries were prepared for sequencing using standard Illumina protocols.

Reference

PMID: https://doi.org/10.3389/fmicb.2019.01174 Title: Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Author: Lingna Lyu, Xiuli Zhang, Cuidan Li, Tingting Yang, Jinghui Wang, Liping Pan, Hongyan Jia, Zihui Li, Qi Sun, Liya Yue, Fei Chen and Zongde Zhang Journal: Front. Microbiol., 28 May 2019 | https://doi.org/10.3389/fmicb.2019.01174 Description: In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC).