exRNAdisease

Entry Detail

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General Information

Database ID: exR0269288 RNA Name: hsa-miR-122-5p RNA Type: miRNA Chromosome: chr18 Starnd: + Coordinate: Start Site(bp): 58451088 End Site(bp): 58451109 External Links: hsa-miR-122-5p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand MSI2 chr17 57255851 57684689 + FAM102A chr9 127940582 127980989 - NAGK chr2 71064344 71079808 + SPRED2 chr2 65310851 65432637 - HGSNAT chr8 43140464 43202855 + PPDPF chr20 63520765 63522206 + SH3GL1 chr19 4360370 4400547 - SETD1A chr16 30957754 30984664 + PEA15 chr1 160205337 160215376 + C19orf54 chr19 40740856 40751553 - PIP4K2A chr10 22534854 22714578 - RPRD1B chr20 38033725 38127780 + CACNG4 chr17 66964707 67033398 + SOX11 chr2 5692384 5701385 + IGFBP5 chr2 216672105 216695549 - RAB1B chr11 66268590 66277492 + CSRP1 chr1 201483530 201509456 - VTI1A chr10 112446998 112818744 + ZNF3 chr7 100064033 100082548 - NHLRC3 chr13 39038306 39050109 + ZNF614 chr19 52012765 52030240 - NDOR1 chr9 137205685 137217009 + ALDOA chr16 30064164 30070457 + GRK3 chr22 25564675 25729294 + C19orf47 chr19 40319536 40348527 - SLC9A1 chr1 27098809 27166981 - STK4 chr20 44966479 45080021 + IER2 chr19 13150411 13154911 + PRR12 chr19 49591182 49626439 + KRT7 chr12 52232520 52252186 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC021092.1 chr19 44103007 44113183 - AC103702.1 chr17 48579630 48582259 - AL358472.3 chr1 153966516 153966930 + AL645465.1 chr1 244375100 244409592 - DSCAM-AS1 chr21 40383083 40385358 + NEAT1 chr11 65422774 65445540 + SNHG7 chr9 136721366 136728184 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.