exRNAdisease

Entry Detail

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General Information

Database ID: exR0269360 RNA Name: hsa-miR-1301-3p RNA Type: miRNA Chromosome: chr2 Starnd: - Coordinate: Start Site(bp): 25328651 End Site(bp): 25328674 External Links: hsa-miR-1301-3p

Disease Information

Disease Name: Multiple Sclerosis Disease Category: Nervous System Diseases MeSH ID: D009103 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Multiple//Sclerosis, Disseminated//Disseminated Sclerosis//MS (Multiple Sclerosis)//Multiple Sclerosis, Acute Fulminating

Expression Detail

GEO ID: GSE140106 Description: Serum exosome microRNAs predict multiple sclerosis disease activity after fingolimod treatment Experimental Design: Fingolimod treatment Case Disease Type: Multiple Sclerosis Case Disease SubType: NA Case Sample: Fingolimod treated Multiple Sclerosis Control Sample: Untreated Number of Case: 27 Number of Control: 27 Number of Samples: 54

Regulatory Relationship

mRNA targets: NA miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0001225 chr22 36684772 36684943 - hsa_circ_0001880 chr9 110084263 110087285 + hsa_circ_0000799 chr17 65941524 65972074 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC015871.3 chr15 79920195 79922455 - AC018521.1 chr17 47945424 47981736 + AC025171.4 chr5 43061395 43062441 + AC067930.1 chr8 143579636 143580670 + AC245033.4 chr15 82533175 82540008 - DANCR chr4 52712404 52720351 + JPX chrX 73944182 74070408 + KCNQ1OT1 chr11 2608328 2699994 - LINC00894 chrX 149938613 150224580 + MUC20-OT1 chr3 195658062 195739964 + NEAT1 chr11 65422774 65445540 + NNT-AS1 chr5 43571594 43603230 - PVT1 chr8 127794526 128187101 + SNHG12 chr1 28578538 28583132 - SNHG16 chr17 76557764 76565348 + Display:

Experiment Detail

GEO ID: GSE140106 Sample Source: Blood Source Fraction: Exosome Platform: GPL11154 Method: NGS Num of detected RNA Type: 1 Num of detected RNAs of this Type: 1907 Sample treatment protocol: Exosomes were isolated from 1 mL RNase A (37 °C for 10 min; 100 ng/ml; Qiagen, Australia) treated serum, by size exclusion chromatography (qEV iZONE Science). RNA Extract protocol: Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. RNA library preparation protocol: Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer's instructions.

Reference

PMID: 31721043 Title: Serum Exosome MicroRNAs Predict Multiple Sclerosis Disease Activity after Fingolimod Treatment Author: Ebrahimkhani S, Beadnall HN, Wang C, Suter CM, Barnett MH, Buckland ME, Vafaee F Journal: Mol Neurobiol. 2020 Feb;57(2):1245-1258. Description: we sought the possible utility of serum exosome microRNAs as biomarkers for disease activity in multiple sclerosis patients in response to fingolimod therapy.