exRNAdisease

Entry Detail

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General Information

Database ID: exR0274536 RNA Name: hsa-miR-147a RNA Type: miRNA Chromosome: chr9 Starnd: - Coordinate: Start Site(bp): 120244985 End Site(bp): 120245004 External Links: hsa-miR-147a

Disease Information

Disease Name: Breast Cancer Disease Category: Cancers MeSH ID: D001943 Type: Neoplasms/Breast Neoplasms Alias: Breast Neoplasms//Breast Neoplasm//Neoplasm, Breast//Breast Tumors//Breast Tumor//Tumor, Breast//Tumors, Breast//Neoplasms, Breast//Breast Cancer//Cancer, Breast//Mammary Cancer//Cancer, Mammary//Cancers, Mammary//Mammary Cancers//Malignant Neoplasm of Breast//Breast Malignant Neoplasm//Breast Malignant Neoplasms//Malignant Tumor of Breast//Breast Malignant Tumor//Breast Malignant Tumors//Cancer of Breast//Cancer of the Breast//Mammary Carcinoma, Human//Carcinoma, Human Mammary//Carcinomas, Human Mammary//Human Mammary Carcinomas//Mammary Carcinomas, Human//Human Mammary Carcinoma//Mammary Neoplasms, Human//Human Mammary Neoplasm//Human Mammary Neoplasms//Neoplasm, Human Mammary//Neoplasms, Human Mammary//Mammary Neoplasm, Human//Breast Carcinoma//Breast Carcinomas//Carcinoma, Breast//Carcinomas, Breast

Expression Detail

GEO ID: GSE22981 Description: Circulating miRNAs as biomarkers and potential functional mediators of early stage breast cancer Experimental Design: Cancer vs Control Case Disease Type: Breast Cancer Case Disease SubType: Early satge breast cancer Case Sample: Breast Cancer Control Sample: Control Number of Case: 20 Number of Control: 20 Number of Samples: 40

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand TWF1 chr12 43793723 43806375 - GLYR1 chr16 4803203 4847288 - PLPP6 chr9 4662294 4665258 + USP37 chr2 218450251 218568351 - CTSD chr11 1752755 1764573 - NUP58 chr13 25301556 25349800 + JARID2 chr6 15246069 15522042 + SCARB1 chr12 124776856 124882668 - MGRN1 chr16 4616493 4690974 + CRKL chr22 20917407 20953747 + SIPA1L3 chr19 37907208 38208369 + TIMP3 chr22 32801701 32863043 + SNAI2 chr8 48917598 48921740 - FAM13A chr4 88725955 89111398 - ZNF618 chr9 113876282 114056591 + SMIM12 chr1 34712737 34859755 - CCND1 chr11 69641156 69654474 + FOXK2 chr17 82519713 82644662 + SLC7A1 chr13 29509414 29595688 - ATP11A chr13 112690329 112887168 + PSME3 chr17 42824385 42843760 + WIZ chr19 15419978 15449956 - PURA chr5 140107777 140125619 + STARD7 chr2 96184859 96208825 - OSBPL9 chr1 51577179 51798427 + PIK3C2A chr11 17077730 17207983 - NKIRAS2 chr17 42011382 42025644 + LIMD1 chr3 45555394 45686341 + TNFRSF10B chr8 23020133 23069031 - ARHGAP35 chr19 46860997 47005077 + miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000816 chr17 80521229 80526077 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004951.4 chr7 43951910 44019151 - AC010442.1 chr5 466124 473098 - AL355987.2 chr9 136800366 136829466 + DSCAM-AS1 chr21 40383083 40385358 + KCNQ1OT1 chr11 2608328 2699994 - MIR503HG chrX 134543119 134546642 - NEAT1 chr11 65422774 65445540 + POLR2J4 chr7 43940895 44019175 - Display:

Experiment Detail

GEO ID: GSE22981 Sample Source: Blood Source Fraction: Plasma Platform: GPL8179 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 801 Sample treatment protocol: NA RNA Extract protocol: Total RNA, including miRNA from plasma, was isolated using the miRNeasy kit (Qiagen) with minor modifications. RNA library preparation protocol: Two hundred ng of total RNA from each sample were labeled and hybridized on Human v2 MicroRNA Expression BeadChips (Cat. no. MI-102-1024; Illumina).

Reference

PMID: 21060830 Title: A pilot study of circulating miRNAs as potential biomarkers of early stage breast cancer Author: Zhao H, Shen J, Medico L, Wang D, Ambrosone CB, Liu SBACKGROUND: To date, there are no highly sensitive and specific minimally invasive biomarkers for detection of breast cancer at an early stage. The occurrence of circulating microRNAs (miRNAs) in blood components (including serum and plasma) has been repeatedly observed in cancer patients as well as healthy controls. Because of the significance of miRNA in carcinogenesis, circulating miRNAs in blood may be unique biomarkers for early and minimally invasive diagnosis of human cancers. The objective of this pilot study was to discover a panel of circulating miRNAs as potential novel breast cancer biomarkers. METHODOLOGY/PRINCIPAL FINDINGS: Using microarray-based expression profiling followed by Real-Time quantitative Polymerase Cycle Reaction (RT-qPCR) validation, we compared the levels of circulating miRNAs in plasma samples from 20 women with early stage breast cancer (10 Caucasian American (CA) and 10 African American (AA)) and 20 matched healthy controls (10 CAs and 10 AAs). Using the significance level of p<0.05 constrained by at least two-fold expression change as selection criteria, we found that 31 miRNAs were differentially expressed in CA study subjects (17 up and 14 down) and 18 miRNAs were differentially expressed in AA study subjects (9 up and 9 down). Interestingly, only 2 differentially expressed miRNAs overlapped between CA and AA study subjects. Using receiver operational curve (ROC) analysis, we show that not only up-regulated but also down-regulated miRNAs can discriminate patients with breast cancer from healthy controls with reasonable sensitivity and specificity. To further explore the potential roles of these circulating miRNAs in breast carcinogenesis, we applied pathway-based bioinformatics exploratory analysis and predicted a number of significantly enriched pathways which are predicted to be regulated by these circulating miRNAs, most of which are involved in critical cell functions, cancer development and progression. CONCLUSIONS: Our observations from this pilot study suggest that the altered levels of circulating miRNAs might have great potential to serve as novel, noninvasive biomarkers for early detection of breast cancer. Journal: PLoS One. 2010 Oct 29;5(10):e13735. Description: The objective of this pilot study was to discover a panel of circulating miRNAs as potential novel breast cancer biomarkers.