exRNAdisease

Entry Detail

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General Information

Database ID: exR0274587 RNA Name: hsa-miR-18a-5p RNA Type: miRNA Chromosome: chr13 Starnd: + Coordinate: Start Site(bp): 91350756 End Site(bp): 91350778 External Links: hsa-miR-18a-5p

Disease Information

Disease Name: Breast Cancer Disease Category: Cancers MeSH ID: D001943 Type: Neoplasms/Breast Neoplasms Alias: Breast Neoplasms//Breast Neoplasm//Neoplasm, Breast//Breast Tumors//Breast Tumor//Tumor, Breast//Tumors, Breast//Neoplasms, Breast//Breast Cancer//Cancer, Breast//Mammary Cancer//Cancer, Mammary//Cancers, Mammary//Mammary Cancers//Malignant Neoplasm of Breast//Breast Malignant Neoplasm//Breast Malignant Neoplasms//Malignant Tumor of Breast//Breast Malignant Tumor//Breast Malignant Tumors//Cancer of Breast//Cancer of the Breast//Mammary Carcinoma, Human//Carcinoma, Human Mammary//Carcinomas, Human Mammary//Human Mammary Carcinomas//Mammary Carcinomas, Human//Human Mammary Carcinoma//Mammary Neoplasms, Human//Human Mammary Neoplasm//Human Mammary Neoplasms//Neoplasm, Human Mammary//Neoplasms, Human Mammary//Mammary Neoplasm, Human//Breast Carcinoma//Breast Carcinomas//Carcinoma, Breast//Carcinomas, Breast

Expression Detail

GEO ID: GSE22981 Description: Circulating miRNAs as biomarkers and potential functional mediators of early stage breast cancer Experimental Design: Cancer vs Control Case Disease Type: Breast Cancer Case Disease SubType: Early satge breast cancer Case Sample: Breast Cancer Control Sample: Control Number of Case: 20 Number of Control: 20 Number of Samples: 40

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand PATZ1 chr22 31325804 31346346 - TUBA4A chr2 219249710 219278170 - SURF4 chr9 133361450 133376166 - DDX5 chr17 64498254 64508199 - SND1 chr7 127652194 128092609 + HIF1AN chr10 100529072 100559998 + SIK2 chr11 111602449 111730855 + FKBP1A chr20 1368978 1393172 - USP10 chr16 84699978 84779922 + PARL chr3 183829271 183884933 - ASF1B chr19 14119512 14136613 - PRPF38B chr1 108692310 108702928 + ADAT2 chr6 143422832 143450695 - ZNF12 chr7 6688433 6706947 - DNAJC9 chr10 73183362 73247255 - AC131160.1 chr3 183815568 183884889 - ACSS2 chr20 34872146 34927962 + MRPS18A chr6 43671303 43687791 - XRCC6 chr22 41621119 41664048 + ROMO1 chr20 35699272 35700984 + PEX5 chr12 7188685 7218574 + NDUFV2 chr18 9102630 9134345 + UBE2Z chr17 48908407 48929056 + NOP58 chr2 202265736 202303661 + RNF4 chr4 2462220 2625320 + CHD1 chr5 98853985 98928957 - ATP2B1 chr12 89588049 89709300 - SLC41A1 chr1 205789094 205813748 - NCDN chr1 35557473 35567274 + HMGCS1 chr5 43287470 43313512 - miRNA targets: NA circRNA targets: circRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa_circ_0000712 chr16 68208276 68225678 + hsa_circ_0001186 chr21 35475133 35475818 + hsa_circ_0001247 chr22 46125304 46136418 + hsa_circ_0000921 chr19 30476129 30477324 + hsa_circ_0000943 chr19 47421744 47440665 + lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC073896.4 chr12 56162359 56190284 - AC093010.3 chr3 114314501 114329714 - AC145207.5 chr17 81878425 81881106 - AL162171.3 chr14 88589231 88592408 + AP005263.1 chr18 9102736 9254346 + CASC2 chr10 118046279 118210158 + GAS5 chr1 173858559 173868882 - NEAT1 chr11 65422774 65445540 + SNHG1 chr11 62851984 62855953 - SNHG15 chr7 44983023 44986961 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE22981 Sample Source: Blood Source Fraction: Plasma Platform: GPL8179 Method: Microarray Num of detected RNA Type: 1 Num of detected RNAs of this Type: 801 Sample treatment protocol: NA RNA Extract protocol: Total RNA, including miRNA from plasma, was isolated using the miRNeasy kit (Qiagen) with minor modifications. RNA library preparation protocol: Two hundred ng of total RNA from each sample were labeled and hybridized on Human v2 MicroRNA Expression BeadChips (Cat. no. MI-102-1024; Illumina).

Reference

PMID: 21060830 Title: A pilot study of circulating miRNAs as potential biomarkers of early stage breast cancer Author: Zhao H, Shen J, Medico L, Wang D, Ambrosone CB, Liu SBACKGROUND: To date, there are no highly sensitive and specific minimally invasive biomarkers for detection of breast cancer at an early stage. The occurrence of circulating microRNAs (miRNAs) in blood components (including serum and plasma) has been repeatedly observed in cancer patients as well as healthy controls. Because of the significance of miRNA in carcinogenesis, circulating miRNAs in blood may be unique biomarkers for early and minimally invasive diagnosis of human cancers. The objective of this pilot study was to discover a panel of circulating miRNAs as potential novel breast cancer biomarkers. METHODOLOGY/PRINCIPAL FINDINGS: Using microarray-based expression profiling followed by Real-Time quantitative Polymerase Cycle Reaction (RT-qPCR) validation, we compared the levels of circulating miRNAs in plasma samples from 20 women with early stage breast cancer (10 Caucasian American (CA) and 10 African American (AA)) and 20 matched healthy controls (10 CAs and 10 AAs). Using the significance level of p<0.05 constrained by at least two-fold expression change as selection criteria, we found that 31 miRNAs were differentially expressed in CA study subjects (17 up and 14 down) and 18 miRNAs were differentially expressed in AA study subjects (9 up and 9 down). Interestingly, only 2 differentially expressed miRNAs overlapped between CA and AA study subjects. Using receiver operational curve (ROC) analysis, we show that not only up-regulated but also down-regulated miRNAs can discriminate patients with breast cancer from healthy controls with reasonable sensitivity and specificity. To further explore the potential roles of these circulating miRNAs in breast carcinogenesis, we applied pathway-based bioinformatics exploratory analysis and predicted a number of significantly enriched pathways which are predicted to be regulated by these circulating miRNAs, most of which are involved in critical cell functions, cancer development and progression. CONCLUSIONS: Our observations from this pilot study suggest that the altered levels of circulating miRNAs might have great potential to serve as novel, noninvasive biomarkers for early detection of breast cancer. Journal: PLoS One. 2010 Oct 29;5(10):e13735. Description: The objective of this pilot study was to discover a panel of circulating miRNAs as potential novel breast cancer biomarkers.