exRNAdisease

Entry Detail

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General Information

Database ID: exR0359182 RNA Name: AACS RNA Type: mRNA Chromosome: chr12 Starnd: + Coordinate: Start Site(bp): 125065434 End Site(bp): 125143333 External Links: ENSG00000081760

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand NPM3 chr10 101781325 101783413 - FGFR2 chr10 121478334 121598458 - PDXK chr21 43719094 43762307 + EXOC6B chr2 72175984 72826041 - SIPA1L2 chr1 232397965 232561558 - NOP14 chr4 2937933 2963406 - ZNF579 chr19 55576774 55580848 - MTERF4 chr2 241072169 241102332 - EIF6 chr20 35278911 35284985 - CITED2 chr6 139371807 139374648 - EME1 chr17 50373220 50381483 + PHLDA1 chr12 76025447 76033932 - RPLP2 chr11 809965 812880 + ZBTB44 chr11 130226677 130314917 - KLHL21 chr1 6590724 6614607 - TMEM259 chr19 1009648 1021179 - RPS5 chr19 58386400 58394806 + PCBP2 chr12 53452102 53481162 + VCP chr9 35056064 35072627 - BRD9 chr5 850291 892801 - TRIM13 chr13 49995888 50020481 + SUPT20H chr13 37009312 37059713 - AIFM2 chr10 70098223 70132934 - AL121753.1 chr20 35267885 35280043 - OST4 chr2 27070472 27071654 - HSP90AB1 chr6 44246166 44253888 + BCKDK chr16 31106107 31112791 + AAMP chr2 218264123 218270257 - SELENOH chr11 57741250 57743554 + BUD31 chr7 99408641 99419616 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC023509.1 chr12 53441741 53467528 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.