exRNAdisease

Entry Detail

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General Information

Database ID: exR0359194 RNA Name: AARS2 RNA Type: mRNA Chromosome: chr6 Starnd: - Coordinate: Start Site(bp): 44298731 End Site(bp): 44313347 External Links: ENSG00000124608

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand COX5A chr15 74919791 74938083 - HSP90AB1 chr6 44246166 44253888 + DENND5A chr11 9138825 9265350 - SFT2D3 chr2 127701497 127705242 + USP22 chr17 20999596 21043760 - SRSF5 chr14 69726900 69772005 + FAM83D chr20 38926312 38953106 + RRP12 chr10 97356358 97426076 - WDR27 chr6 169457212 169702067 - KPNB1 chr17 47649476 47685505 + RAD23A chr19 12945855 12953642 + RBM8A chr1 145917714 145927678 - TIMELESS chr12 56416363 56449426 - MIDN chr19 1248553 1259140 + MT-ND2 chrMT 4470 5511 + GRAMD1A chr19 34994784 35026471 + TM9SF3 chr10 96518110 96587452 - TP53I11 chr11 44885903 44951306 - HSP90B1 chr12 103930107 103953645 + NDFIP1 chr5 142108779 142154440 + USP35 chr11 78188812 78215232 + RPL13 chr16 89560657 89566828 + EIF3C chr16 28688558 28735730 + DPPA4 chr3 109326141 109337572 - ARSA chr22 50622754 50628173 - TBL1XR1 chr3 177019340 177228000 - WDR74 chr11 62832342 62841809 - ORAI3 chr16 30949068 30956461 + PTOV1 chr19 49850735 49860744 + PML chr15 73994673 74047827 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC102953.2 chr7 1464497 1467522 - AL355075.4 chr14 20343048 20343685 - EBLN3P chr9 37079645 37090928 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.