exRNAdisease

Entry Detail

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General Information

Database ID: exR0359196 RNA Name: AASDH RNA Type: mRNA Chromosome: chr4 Starnd: - Coordinate: Start Site(bp): 56338287 End Site(bp): 56387508 External Links: ENSG00000157426

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand CANT1 chr17 78991716 79009867 - EMC7 chr15 34084017 34101862 - EPN1 chr19 55675226 55709858 + GAPDH chr12 6534512 6538374 + GK3P chr4 165277812 165279679 - GOLPH3 chr5 32124716 32174319 - HMGCR chr5 75336329 75362101 + HOXB1 chr17 48528526 48531011 - LARP6 chr15 70829130 70854157 - MAST3 chr19 18097793 18151692 + MGST1 chr12 16347142 16609259 + MTHFD2 chr2 74198610 74217565 + MTRNR2L12 chr3 96617188 96618236 - NDUFS5 chr1 39026318 39034636 + PIP5K1A chr1 151197949 151249536 + PLCXD1 chrX 276322 303356 + RBFOX2 chr22 35738736 36028824 - RER1 chr1 2391775 2405442 + RPS18 chr6 33272075 33276511 + SLC11A2 chr12 50979401 51028566 - SPAG9 chr17 50962174 51120868 - TFPI chr2 187464230 187565760 - TPP1 chr11 6612768 6619448 - UBE2I chr16 1308880 1327018 + UGP2 chr2 63840940 63891562 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC012485.1 chr2 238231684 238255633 + NAALADL2-AS2 chr3 175234861 175271096 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.