exRNAdisease

Entry Detail

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General Information

Database ID: exR0359200 RNA Name: ABCA1 RNA Type: mRNA Chromosome: chr9 Starnd: - Coordinate: Start Site(bp): 104781006 End Site(bp): 104928155 External Links: ENSG00000165029

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand COX5B chr2 97646062 97648383 + EIF3L chr22 37848868 37889407 + EWSR1 chr22 29268009 29300525 + FEN1 chr11 61792911 61797238 + GANAB chr11 62624826 62646726 - GAPDH chr12 6534512 6538374 + HADH chr4 107989714 108035175 + IGF2 chr11 2129112 2141238 - IGF2BP3 chr7 23310209 23470491 - KDM3B chr5 138352685 138437028 + LSMEM1 chr7 112480853 112491062 + LUC7L3 chr17 50719565 50756219 + MPI chr15 74890005 74902219 + NISCH chr3 52455118 52493068 + PIANP chr12 6693792 6700800 - PPRC1 chr10 102132994 102150333 + RPL13A chr19 49487554 49492308 + RPL14 chr3 40457292 40468587 + RPL19 chr17 39200283 39204732 + RPS23 chr5 82273320 82278396 - SEC11A chr15 84669538 84716460 - SHROOM3 chr4 76435229 76783253 + SND1 chr7 127652194 128092609 + UBE2R2 chr9 33817160 33920399 + ZNF217 chr20 53567065 53609907 - ZNF568 chr19 36916329 36998700 + ZNF696 chr8 143289676 143299952 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-1294 chr5 154347153 154347174 + circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.