exRNAdisease

Entry Detail

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General Information

Database ID: exR0359213 RNA Name: ABCB4 RNA Type: mRNA Chromosome: chr7 Starnd: - Coordinate: Start Site(bp): 87401697 End Site(bp): 87480435 External Links: ENSG00000005471

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCB1 chr7 87503017 87713323 - AP1G2 chr14 23559565 23568070 - BASP1 chr5 17065598 17276843 + CENPV chr17 16342534 16353656 - CLDN6 chr16 3014712 3020071 - CUL5 chr11 108008898 108107761 + DDX5 chr17 64498254 64508199 - EIF5A chr17 7306999 7312463 + FXR2 chr17 7591230 7614897 - HNRNPDL chr4 82422564 82430408 - HSPA8 chr11 123057489 123063230 - HTATSF1 chrX 136497079 136512346 + KIF5B chr10 32009015 32056425 - MAF1 chr8 144104461 144107611 + NUAK1 chr12 106063340 106140033 - PAM chr5 102753981 103031105 + PHF14 chr7 10973336 11169630 + RPL5 chr1 92832013 92841924 + SERF2 chr15 43777087 43802589 + SLC39A1 chr1 153959099 153968184 - SNX18 chr5 54517759 54546586 + SPOCK2 chr10 72059034 72089032 - STX7 chr6 132445867 132513198 - TMEM167A chr5 83052846 83077863 - TPT1 chr13 45333471 45341370 - ZNF136 chr19 12163064 12189871 + ZNF385A chr12 54369133 54391298 - miRNA targets: NA circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.