exRNAdisease

Entry Detail

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General Information

Database ID: exR0359225 RNA Name: ABCC5 RNA Type: mRNA Chromosome: chr3 Starnd: - Coordinate: Start Site(bp): 183919934 End Site(bp): 184017939 External Links: ENSG00000114770

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand VAPA chr18 9914002 9960021 + DGKQ chr4 958887 986895 - STRADA chr17 63682336 63741986 - POFUT1 chr20 32207880 32238658 + DSTN chr20 17570075 17609919 + EEF1D chr8 143579697 143599541 - STRA6 chr15 74179466 74212267 - WDR43 chr2 28894667 28948219 + TMEM63A chr1 225845536 225882380 - OBSL1 chr2 219550728 219571859 - RITA1 chr12 113185526 113192368 + MKRN1 chr7 140453033 140479536 - BSG chr19 571277 583493 + CALM3 chr19 46601074 46610782 + RPL37 chr5 40825262 40835222 - PLEKHG3 chr14 64704102 64750249 + SMAD4 chr18 51028394 51085045 + SNX5 chr20 17941597 17968980 - PPP2R3B chrX 333933 386955 - SCMH1 chr1 41027200 41242154 - LCK chr1 32251239 32286165 + SIK2 chr11 111602449 111730855 + PPP2R1B chr11 111726908 111766389 - SNX2 chr5 122775079 122834543 + KLHL23 chr2 169694488 169776989 + RAB4B-EGLN2 chr19 40778242 40808418 + WDR86 chr7 151375909 151410727 - REPS1 chr6 138903493 138988261 - PABPC3 chr13 25095868 25099254 + PROCA1 chr17 28703197 28711854 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-185-5p chr22 20033153 20033174 + hsa-miR-423-5p chr17 30117095 30117117 + hsa-miR-1323 chr19 53671978 53671999 + hsa-miR-548o-3p chr20 38516607 38516628 + circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.