exRNAdisease

Entry Detail

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General Information

Database ID: exR0359255 RNA Name: ABI2 RNA Type: mRNA Chromosome: chr2 Starnd: + Coordinate: Start Site(bp): 203328239 End Site(bp): 203447728 External Links: ENSG00000138443

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand C15orf39 chr15 75195643 75212169 + KLC1 chr14 103561896 103714249 + PGAM1 chr10 97426191 97433444 + ESPL1 chr12 53268299 53293638 + COX5B chr2 97646062 97648383 + MYH9 chr22 36281277 36388067 - YWHAQ chr2 9583967 9630997 - HLA-DQA1 chr6 32628179 32647062 + MAN1B1 chr9 137086985 137109183 + PUDP chrX 6667865 7148190 - PEX19 chr1 160276812 160286348 - PKM chr15 72199029 72231822 - TRAF7 chr16 2155698 2178129 + DICER1 chr14 95086228 95158010 - LAMTOR4 chr7 100148907 100155944 + ATN1 chr12 6924463 6942321 + LMNB1 chr5 126776623 126837020 + AL139300.1 chr14 103562962 103685924 + SLC15A4 chr12 128793194 128823958 - CCT2 chr12 69585426 69601570 + NPLOC4 chr17 81556887 81648465 - MT-CO3 chrMT 9207 9990 + SLC9A3R1 chr17 74748628 74769353 + API5 chr11 43311963 43344529 + HSDL2 chr9 112380080 112472405 + BOLA2-SMG1P6 chr16 29443230 29454651 - TXNDC5 chr6 7881517 7910788 - C9orf40 chr9 74946583 74952912 - RPL22L1 chr3 170864875 170870208 - HYOU1 chr11 119044188 119057227 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-218-5p chr5 168768211 168768231 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC079354.2 chr2 202178660 202179391 - ARHGAP27P1-BPTFP1-KPNA2P3 chr17 64749663 64781707 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.