exRNAdisease

Entry Detail

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General Information

Database ID: exR0359284 RNA Name: ACAP2 RNA Type: mRNA Chromosome: chr3 Starnd: - Coordinate: Start Site(bp): 195274745 End Site(bp): 195443044 External Links: ENSG00000114331

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand SERPINB5 chr18 63476958 63505085 + RPS23 chr5 82273320 82278396 - MTRNR2L12 chr3 96617188 96618236 - KRT8 chr12 52897187 52949954 - TMEM63A chr1 225845536 225882380 - CDCA7L chr7 21900899 21945903 - SSBP4 chr19 18418864 18434562 + RAB34 chr17 28714281 28718429 - ATG4C chr1 62784132 62865516 + MT-ND3 chrMT 10059 10404 + CYFIP2 chr5 157266079 157395595 + DCTN6 chr8 30156319 30183639 + ACAP3 chr1 1292390 1309609 - CREB1 chr2 207529737 207605988 + CLU chr8 27596917 27614700 - COQ4 chr9 128322544 128334072 + ZFR chr5 32354350 32444740 - FLVCR1 chr1 212858275 212899363 + HLA-DRA chr6 32439878 32445046 + ACOT1 chr14 73537143 73543796 + ANAPC15 chr11 72106378 72112780 - SBNO1 chr12 123289109 123364847 - AAK1 chr2 69457997 69674349 - C20orf204 chr20 64034344 64039962 + HSD17B11 chr4 87336515 87391188 - PRKCA chr17 66302613 66810743 + MT-CO3 chrMT 9207 9990 + LCP1 chr13 46125920 46211871 - NRDC chr1 51789191 51878937 - ERP29 chr12 112013348 112023449 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-320a-3p chr8 22244975 22244996 - hsa-miR-320b chr1 224257049 224257070 - hsa-miR-320c chr18 24321716 24321735 + hsa-miR-320d chr13 40727828 40727846 - circRNA targets: NA lncRNA targets: NA Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.