exRNAdisease

Entry Detail

---



General Information

Database ID: exR0359298 RNA Name: ACLY RNA Type: mRNA Chromosome: chr17 Starnd: - Coordinate: Start Site(bp): 41866917 End Site(bp): 41930542 External Links: ENSG00000131473

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AC093155.3 chr1 86993009 87169204 + CTCF chr16 67562467 67639177 + TRMT6 chr20 5937228 5950558 - ADAMTSL4 chr1 150549369 150560937 + PHF1 chr6 33410399 33416453 + LY6E chr8 143017982 143023832 + NOLC1 chr10 102152176 102163871 + CD276 chr15 73683966 73714514 + HSBP1 chr16 83807978 83819737 + INS-IGF2 chr11 2132538 2161209 - RAB8A chr19 16111889 16134234 + HSPA8 chr11 123057489 123063230 - RUVBL2 chr19 48993562 49015970 + ACBD3 chr1 226144679 226186741 - DCAF5 chr14 69050881 69153150 - OAZ1 chr19 2269509 2273490 + ZC3H13 chr13 45954465 46052759 - RNF115 chr1 145738868 145824095 - EEF1D chr8 143579697 143599541 - MTOR chr1 11106535 11262551 - LCOR chr10 96832282 96995959 + TNIP1 chr5 151029945 151093577 - OS9 chr12 57693955 57721557 + IGF2 chr11 2129112 2141238 - HMGA1 chr6 34236873 34246231 + SREBF2 chr22 41833079 41907307 + WDR33 chr2 127701027 127811187 - ATIC chr2 215311956 215349773 + EPHB4 chr7 100802565 100827523 - GUK1 chr1 228139962 228148984 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-22-3p chr17 1713914 1713935 - hsa-miR-27a-3p chr19 13836447 13836467 - hsa-miR-324-3p chr17 7223308 7223330 - hsa-miR-1269a chr4 66276890 66276911 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007952.4 chr17 19112000 19112636 - AC019205.1 chr6 73263212 73301789 + AL121899.1 chr20 2207217 2213151 + AL132655.2 chr20 58817132 58817725 - AC138761.1 chr17 22266395 22288301 + AL355075.4 chr14 20343048 20343685 - LINC01356 chr1 112820170 112850643 - PURPL chr5 27217714 27496994 + TERC chr3 169764520 169765060 - TMEM161B-AS1 chr5 88268891 88439337 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.