exRNAdisease

Entry Detail

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General Information

Database ID: exR0359303 RNA Name: ACOT13 RNA Type: mRNA Chromosome: chr6 Starnd: + Coordinate: Start Site(bp): 24667035 End Site(bp): 24705065 External Links: ENSG00000112304

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand CCT3 chr1 156308968 156367873 - CDC26 chr9 113255835 113275572 - PARP1 chr1 226360691 226408093 - MTRNR2L8 chr11 10507894 10509186 - NDRG4 chr16 58462846 58513628 + TMEM97 chr17 28319200 28328685 + CBX5 chr12 54230942 54280133 - RAF1 chr3 12583601 12664226 - ZBTB25 chr14 64449106 64505213 - SLC16A3 chr17 82228397 82261129 + FUT4 chr11 94543840 94549898 + TRIM69 chr15 44728988 44767829 + RPS15 chr19 1438358 1440494 + HNRNPUL2-BSCL2 chr11 62690275 62727384 - RPRD1A chr18 35984387 36067576 - EEF1A1 chr6 73515750 73523797 - HNRNPUL2 chr11 62712630 62727457 - AL121594.1 chr14 35122549 35317474 + PRPF38A chr1 52404602 52420836 + FZD3 chr8 28494205 28574267 + SMPDL3A chr6 122789049 122809720 + CALM3 chr19 46601074 46610782 + EFNA1 chr1 155127876 155134899 + EI24 chr11 125569280 125584684 + RND2 chr17 43025231 43032041 + TAF15 chr17 35713791 35864615 + PRRC1 chr5 127517609 127555089 + KDM1A chr1 23019443 23083689 + MSC chr8 71841549 71844468 - MFSD5 chr12 53251251 53254405 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-92a-3p chrX 134169544 134169565 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand MIR17HG chr13 91347820 91354579 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.