exRNAdisease

Entry Detail

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General Information

Database ID: exR0359327 RNA Name: ACSS3 RNA Type: mRNA Chromosome: chr12 Starnd: + Coordinate: Start Site(bp): 80936414 End Site(bp): 81261210 External Links: ENSG00000111058

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand TRAM1L1 chr4 117083554 117085576 - NMNAT2 chr1 183248237 183418380 - EPB41L2 chr6 130839347 131063322 - CNST chr1 246566444 246668595 + TJP1 chr15 29699367 29968865 - UBL7 chr15 74445977 74461182 - SENP3-EIF4A1 chr17 7563287 7578715 + UBE2E1 chr3 23805955 23891640 + MIDN chr19 1248553 1259140 + TUBB chr6 30720352 30725426 + KXD1 chr19 18557762 18569387 + U2SURP chr3 142964497 143060725 + TPI1 chr12 6867119 6870948 + UBE2D2 chr5 139526431 139628434 + SCAPER chr15 76347904 76905444 - PTGES3 chr12 56663341 56688408 - DEK chr6 18223860 18264548 - OPRD1 chr1 28812142 28871267 + POLR1D chr13 27620742 27744237 + RPS23 chr5 82273320 82278396 - EIF4A1 chr17 7572706 7579006 + RHEB chr7 151466012 151520120 - RPS6KC1 chr1 213051233 213274774 + ME2 chr18 50879080 50954257 + ARF4 chr3 57571363 57598220 - ENO1 chr1 8861000 8879190 - SWT1 chr1 185157080 185291781 + CRABP2 chr1 156699606 156705816 - NONO chrX 71283192 71301168 + HMGN4 chr6 26538366 26546933 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC016876.2 chr17 7581964 7584086 - NIFK-AS1 chr2 121649320 121728563 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.