exRNAdisease

Entry Detail

---



General Information

Database ID: exR0359337 RNA Name: ACTN1 RNA Type: mRNA Chromosome: chr14 Starnd: - Coordinate: Start Site(bp): 68874143 End Site(bp): 68979440 External Links: ENSG00000072110

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand SLCO3A1 chr15 91853708 92172435 + ATP5F1B chr12 56638175 56645984 - MT-CO2 chrMT 7586 8269 + ZNF865 chr19 55605405 55617269 + DPM2 chr9 127935099 127938484 - G3BP1 chr5 151771045 151812785 + ACTN3 chr11 66546395 66563334 + VAT1 chr17 43014607 43025123 - HMGN1 chr21 39342315 39349647 - SUPT16H chr14 21351476 21384019 - PLPP3 chr1 56494761 56645301 - ERG28 chr14 75649791 75660876 - PKM chr15 72199029 72231822 - SPIN2B chrX 57118551 57121546 - NPC2 chr14 74476192 74494177 - PFKL chr21 44300051 44327376 + FYN chr6 111660332 111873452 - FARP2 chr2 241356285 241494841 + TRAF7 chr16 2155698 2178129 + AC245033.1 chr15 82536788 82573194 - SEC11A chr15 84669538 84716460 - HLA-B chr6 31269491 31357188 - DGCR8 chr22 20080232 20111877 + CHTF8 chr16 69118010 69132578 - ITFG2 chr12 2812622 2859791 + POLL chr10 101578882 101588270 - POLB chr8 42338454 42371808 + ROCK1 chr18 20946906 21111813 - CNOT1 chr16 58519951 58629885 - PITPNA chr17 1517718 1562792 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-367-3p chr4 112647877 112647898 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC245033.4 chr15 82533175 82540008 - BX284668.5 chr1 16887577 16889706 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.