exRNAdisease

Entry Detail

---



General Information

Database ID: exR0359339 RNA Name: ACTN4 RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 38647649 End Site(bp): 38731589 External Links: ENSG00000130402

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand NUP58 chr13 25301556 25349800 + ID1 chr20 31605283 31606515 + SAPCD1 chr6 31762996 31764851 + SNRNP200 chr2 96274338 96321271 - HSPA1A chr6 31815543 31817946 + MAN1C1 chr1 25617468 25786207 + IREB2 chr15 78437431 78501453 + CIB2 chr15 78104606 78131535 - CRNKL1 chr20 20034368 20056046 - TMEM158 chr3 45224466 45226287 - AC020915.5 chr19 58228914 58315183 + GCNT1 chr9 76419850 76651203 + ATIC chr2 215311956 215349773 + VAPA chr18 9914002 9960021 + YWHAQ chr2 9583967 9630997 - CHD9 chr16 53055033 53329150 + LRRD1 chr7 92141643 92179531 - RAD23B chr9 107283137 107332192 + CFL1 chr11 65823022 65862026 - STX10 chr19 13144058 13150383 - PRRC2B chr9 131373636 131500197 + MTRNR2L8 chr11 10507894 10509186 - HNRNPA2B1 chr7 26173057 26201529 - BSPRY chr9 113349541 113371233 + ZFHX4 chr8 76681239 76867281 + PTPN6 chr12 6946468 6961316 + SET chr9 128683424 128696400 + NEDD8 chr14 24216857 24232367 - PHLDB3 chr19 43474954 43504935 - PKM chr15 72199029 72231822 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-138-5p chr16 56858527 56858549 + hsa-miR-185-5p chr22 20033153 20033174 + hsa-miR-296-5p chr20 58817661 58817681 - hsa-miR-654-5p chr14 101040234 101040255 + hsa-miR-423-5p chr17 30117095 30117117 + hsa-miR-483-5p chr11 2134181 2134202 - hsa-miR-1286 chr22 20249145 20249165 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC010997.3 chr10 75279726 75401246 - AC016876.2 chr17 7581964 7584086 - AC026304.1 chr3 143000907 143001467 - AC132217.1 chr11 2129121 2129964 - AC245014.3 chr1 145281116 145281462 + AC245033.4 chr15 82533175 82540008 - AD000090.1 chr19 35557956 35581954 + AC008731.1 chr16 24803451 24819739 - AC020915.1 chr19 58278966 58315197 + AC093734.1 chr7 1508655 1509427 - AC135178.2 chr17 8318088 8318712 - AL355075.4 chr14 20343048 20343685 - GHRLOS chr3 10285754 10294903 + LIMD1-AS1 chr3 45676369 45689200 - RMRP chr9 35657751 35658018 - SLC9A3-AS1 chr5 473236 480884 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.