exRNAdisease

Entry Detail

---



General Information

Database ID: exR0359341 RNA Name: ACTR1A RNA Type: mRNA Chromosome: chr10 Starnd: - Coordinate: Start Site(bp): 102461881 End Site(bp): 102502712 External Links: ENSG00000138107

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACTR1B chr2 97655939 97664044 - AP002990.1 chr11 62559603 62591531 - PPP3R1 chr2 68178857 68256237 - MT-ND3 chrMT 10059 10404 + CDK19 chr6 110609978 110815958 - ERC2 chr3 55508311 56468467 - MT-ND1 chrMT 3307 4262 + INO80 chr15 40978880 41116280 - TCOF1 chr5 150357629 150400308 + OS9 chr12 57693955 57721557 + GOLGA7 chr8 41490396 41510980 + PAPOLA chr14 96501433 96567111 + IGFBP2 chr2 216632828 216664436 + RPL27A chr11 8682788 8714759 + PDE7A chr8 65717510 65842322 - RPS6 chr9 19375715 19380236 - HNRNPL chr19 38836388 38852347 - MT-ND4 chrMT 10760 12137 + EIF5AL1 chr10 79512601 79516440 + COMMD10 chr5 116085016 116412762 + TMSB4X chrX 12975110 12977227 + RPL29 chr3 51993522 51995895 - CBSL chr21 6444869 6468040 - GGCT chr7 30496621 30504841 - ARHGEF19 chr1 16197854 16212652 - SAE1 chr19 47113274 47210636 + TRIM28 chr19 58544064 58550722 + CHST14 chr15 40470998 40474571 + TBCB chr19 36114289 36125947 + CREBL2 chr12 12611827 12645108 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-363-3p chrX 134169382 134169403 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC011297.1 chrY 6918682 6920791 + AC027020.2 chr15 100547765 100550153 - AC245014.3 chr1 145281116 145281462 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.