exRNAdisease

Entry Detail

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General Information

Database ID: exR0359343 RNA Name: ACTR2 RNA Type: mRNA Chromosome: chr2 Starnd: + Coordinate: Start Site(bp): 65227753 End Site(bp): 65271253 External Links: ENSG00000138071

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand TBPL1 chr6 133952170 133990432 + LEPROT chr1 65420587 65436007 + SHMT2 chr12 57229573 57234935 + DDX10 chr11 108665069 108940927 + VASH2 chr1 212950520 212992037 + FUS chr16 31180110 31194871 + FAAP100 chr17 81539885 81553961 - KIAA0355 chr19 34254552 34355571 + WASF2 chr1 27404230 27490167 - TUBA3D chr2 131476119 131482934 + UBA52 chr19 18571730 18577550 + SURF6 chr9 133328776 133336188 - AMOT chrX 112774503 112840815 - RBM47 chr4 40423267 40630875 - TBC1D7 chr6 13266542 13328583 - GLS chr2 190880821 190965552 + PRMT5 chr14 22920525 22929408 - IGF2 chr11 2129112 2141238 - GZF1 chr20 23362182 23373062 + UCHL5 chr1 193012250 193060080 - VTA1 chr6 142147162 142224685 + MXI1 chr10 110207605 110287365 + FRMD8 chr11 65386621 65413525 + RTN3 chr11 63681446 63759891 + KPNA1 chr3 122421902 122514945 - AL035078.4 chr11 31812391 32104665 + MTRNR2L12 chr3 96617188 96618236 - RAC2 chr22 37225270 37244448 - RBM8A chr1 145917714 145927678 - ATP9B chr18 79069285 79378287 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC068831.7 chr15 91022766 91023200 + AC138761.1 chr17 22266395 22288301 + LINC01018 chr5 6582136 6600288 + LINC01641 chr1 227393554 227431035 + OIP5-AS1 chr15 41283990 41309737 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.