exRNAdisease

Entry Detail

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General Information

Database ID: exR0359362 RNA Name: ADAM10 RNA Type: mRNA Chromosome: chr15 Starnd: - Coordinate: Start Site(bp): 58588809 End Site(bp): 58749791 External Links: ENSG00000137845

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand RPS11 chr19 49496365 49499708 + THUMPD3 chr3 9362971 9386791 + ATXN3 chr14 92044496 92106621 - NUDT21 chr16 56429133 56452199 - MORF4L1 chr15 78810487 78898139 + RPS24 chr10 78033760 78056813 + SMARCC1 chr3 47585269 47782106 - YY1 chr14 100238298 100282788 + RB1CC1 chr8 52622458 52745843 - SPATS2L chr2 200305881 200482264 + MAPK9 chr5 180233143 180292099 - RFLNA chr12 123973241 124316024 + TCTN3 chr10 95663396 95694143 - RPL13 chr16 89560657 89566828 + MRPL30 chr2 99181152 99199561 + METTL2B chr7 128476729 128506602 + MPZL1 chr1 167721192 167791919 + OSBP chr11 59574398 59615774 - PBX1 chr1 164555584 164899296 + TAB2 chr6 149218641 149411613 + RBM42 chr19 35629030 35637686 + WBP11 chr12 14784582 14803486 - TMEM189 chr20 50118254 50153734 - TRIM28 chr19 58544064 58550722 + GNAI3 chr1 109548615 109600195 + ANXA6 chr5 151100706 151157785 - XRCC6 chr22 41621119 41664048 + PGM3 chr6 83161150 83193936 - ACTN4 chr19 38647649 38731589 + E2F2 chr1 23506438 23531233 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-1248 chr3 186786675 186786701 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL096870.2 chr14 24209646 24215987 - AC068790.8 chr12 123973215 124088594 + AL645465.1 chr1 244375100 244409592 - FGD5-AS1 chr3 14920347 14948424 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.