exRNAdisease

Entry Detail

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General Information

Database ID: exR0359380 RNA Name: ADAMTS13 RNA Type: mRNA Chromosome: chr9 Starnd: + Coordinate: Start Site(bp): 133414358 End Site(bp): 133459402 External Links: ENSG00000160323

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ATXN10 chr22 45671798 45845307 + BCR chr22 23179704 23318037 + CCDC124 chr19 17933015 17943991 + DDX24 chr14 94048287 94081212 - EME2 chr16 1772810 1781708 + ESYT1 chr12 56118250 56144671 + GCN1 chr12 120127202 120194715 - GDI2 chr10 5765223 5842132 - GNS chr12 64713445 64759431 - LUC7L chr16 188969 229463 - MAPK9 chr5 180233143 180292099 - MARCKSL1 chr1 32333839 32336233 - NDUFV1 chr11 67605653 67612554 + PDXK chr21 43719094 43762307 + PLEKHJ1 chr19 2230084 2237704 - PPP2R1A chr19 52190048 52229518 + PTOV1 chr19 49850735 49860744 + PTPN11 chr12 112418351 112509913 + SPG11 chr15 44562696 44663678 - SPG7 chr16 89490719 89557768 + SUV39H1 chrX 48695554 48709012 + WDR34 chr9 128633661 128656787 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC121761.1 chr12 75483454 75489820 - AC124068.2 chr15 89335053 89336161 + Z95331.1 chr22 45657019 45680130 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.