exRNAdisease

Entry Detail

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General Information

Database ID: exR0359404 RNA Name: ADAT3 RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 1905399 End Site(bp): 1913447 External Links: ENSG00000213638

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand STRN3 chr14 30893799 31026401 - DBN1 chr5 177456608 177474401 - NUDT19 chr19 32691961 32713796 + STARD9 chr15 42575606 42720998 + KRT18 chr12 52948871 52952906 + JUND chr19 18279694 18281622 - MLLT6 chr17 38705273 38729795 + TMEM256-PLSCR3 chr17 7389734 7404097 - LARP1 chr5 154712843 154817605 + SYMPK chr19 45815410 45863194 - SLC9A3R1 chr17 74748628 74769353 + EXOSC2 chr9 130693721 130704894 + TMEM132A chr11 60924463 60937159 + HNRNPAB chr5 178204533 178211163 + DHX38 chr16 72093613 72112912 + HNRNPK chr9 83968083 83980616 - PLSCR3 chr17 7389727 7394842 - RPS28 chr19 8321158 8323340 + C5AR1 chr19 47290023 47322066 + DZIP1 chr13 95578202 95644703 - CIC chr19 42268537 42295797 + ZNF721 chr4 425815 499156 - SRSF4 chr1 29147743 29181900 - SIRPA chr20 1894167 1940592 + SCAMP4 chr19 1905372 1926013 + ARAP1 chr11 72685069 72793599 - RAN chr12 130872037 130877678 + HTT chr4 3041422 3243960 + PKMYT1 chr16 2968024 2980479 - CYB5R3 chr22 42617840 42649392 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand MIRLET7BHG chr22 46053869 46113928 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.