exRNAdisease

Entry Detail

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General Information

Database ID: exR0359417 RNA Name: ADD2 RNA Type: mRNA Chromosome: chr2 Starnd: - Coordinate: Start Site(bp): 70607618 End Site(bp): 70768225 External Links: ENSG00000075340

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand COL21A1 chr6 56056590 56394094 - PRKD3 chr2 37250502 37324808 - SMIM20 chr4 25861830 25929874 + CNN3 chr1 94896949 94927223 - SHISA5 chr3 48467798 48504826 - PRUNE1 chr1 151008420 151035713 + FUBP3 chr9 130578965 130638352 + YWHAB chr20 44885702 44908532 + USP40 chr2 233475520 233566782 - DYNC1H1 chr14 101964573 102056443 + YIPF4 chr2 32277904 32316594 + RPS6 chr9 19375715 19380236 - C12orf75 chr12 105235290 105396097 + AC138894.1 chr16 28456371 28492098 - PPID chr4 158709127 158723396 - P4HB chr17 81843161 81860624 - CMSS1 chr3 99817837 100181732 + HSP90AB1 chr6 44246166 44253888 + TREX2 chrX 153444720 153470587 - AAK1 chr2 69457997 69674349 - RPL11 chr1 23691742 23696835 + MAP3K3 chr17 63622415 63696303 + ANKRD13C chr1 70258999 70354734 - ATP5F1D chr19 1241746 1244825 + GDF11 chr12 55743122 55757264 + ZMIZ2 chr7 44748581 44769881 + SRSF9 chr12 120461672 120469748 - SHMT1 chr17 18327860 18363563 - RPL4 chr15 66498015 66524532 - GTPBP1 chr22 38705742 38738299 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC245014.3 chr1 145281116 145281462 + AL356489.2 chr9 33697459 33700986 + FOXD3-AS1 chr1 63320884 63324441 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.