exRNAdisease

Entry Detail

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General Information

Database ID: exR0359423 RNA Name: ADH5 RNA Type: mRNA Chromosome: chr4 Starnd: - Coordinate: Start Site(bp): 99070978 End Site(bp): 99088801 External Links: ENSG00000197894

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand GALNT2 chr1 230057990 230282122 + CLPTM1 chr19 44954585 44993341 + BUB1B chr15 40161023 40221136 + MAD1L1 chr7 1815793 2233243 - PSAP chr10 71816298 71851325 - RSAD1 chr17 50478860 50485974 + PTPN6 chr12 6946468 6961316 + ACTR6 chr12 100199122 100241865 + SRP68 chr17 76038775 76072517 - CEBPZ chr2 37201612 37231596 - SEC61A1 chr3 128051641 128071683 + TYRO3 chr15 41557675 41583589 + RPS19 chr19 41860255 41872925 + SMARCA4 chr19 10960825 11079426 + ILF2 chr1 153661788 153671028 - NUDCD3 chr7 44379119 44490658 - SEC22A chr3 123201927 123274136 + RAN chr12 130872037 130877678 + OTX2 chr14 56799905 56816693 - RPL15 chr3 23916545 23924374 + SLX1A chr16 30193887 30197561 + C5orf30 chr5 103258763 103278660 + TUBB chr6 30720352 30725426 + WDFY1 chr2 223855716 223945357 - AL136454.1 chr1 192716132 192716653 + VIM chr10 17228241 17237593 + TAF6 chr7 100107070 100119841 - HMGA1 chr6 34236873 34246231 + PABPC1 chr8 100685816 100722809 - VCL chr10 73995193 74121363 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-1825 chr20 32237830 32237847 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand C5orf17 chr5 23951348 24178263 + OXCT1-AS1 chr5 41869927 41872241 + SLX1A-SULT1A3 chr16 30193892 30204310 + SNHG15 chr7 44983023 44986961 - TUG1 chr22 30969245 30979395 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.