exRNAdisease

Entry Detail

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General Information

Database ID: exR0359437 RNA Name: ADORA2A RNA Type: mRNA Chromosome: chr22 Starnd: + Coordinate: Start Site(bp): 24417879 End Site(bp): 24442357 External Links: ENSG00000128271

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand TUBA1A chr12 49184795 49189080 - ILK chr11 6603708 6610874 + RPL23A chr17 28719985 28724359 + TUB chr11 8019244 8106112 + GABARAP chr17 7240008 7242449 - RETREG2 chr2 219176225 219185475 + RPS3 chr11 75399515 75422280 + LMNA chr1 156082573 156140089 + SPECC1L chr22 24270817 24417739 + IFI30 chr19 18173162 18178117 + NFKB2 chr10 102394110 102402524 + RPL9 chr4 39452521 39458949 - MX1 chr21 41420304 41459214 + EIF4B chr12 53006158 53042209 + LRFN4 chr11 66856647 66860475 + CTSH chr15 78921058 78949574 - CHCHD3 chr7 132784870 133082090 - ITGB1 chr10 32900318 33005792 - MTPN chr7 135926760 135977359 - IFFO1 chr12 6538375 6556083 - CLK3 chr15 74598500 74645414 + RPL3 chr22 39312882 39320389 - P4HB chr17 81843161 81860624 - RPS16 chr19 39433137 39435949 - VAMP5 chr2 85584431 85593406 + SPECC1L-ADORA2A chr22 24270898 24442356 + TMSB4X chrX 12975110 12977227 + MCM2 chr3 127598410 127622436 + ACTG1 chr17 81509971 81523847 - EIF3D chr22 36510855 36529184 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL121832.3 chr20 62402236 62405935 - AL137127.1 chr1 19072110 19075511 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.