exRNAdisease

Entry Detail

---



General Information

Database ID: exR0359458 RNA Name: AFAP1 RNA Type: mRNA Chromosome: chr4 Starnd: - Coordinate: Start Site(bp): 7758714 End Site(bp): 7939926 External Links: ENSG00000196526

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand RAPGEF1 chr9 131576770 131740074 - PGAM1 chr10 97426191 97433444 + RBX1 chr22 40951347 40973309 + ZNF462 chr9 106863166 107013634 + DKC1 chrX 154762742 154777689 + PA2G4 chr12 56104537 56113910 + NCL chr2 231453531 231483641 - LAMTOR2 chr1 156054782 156058506 + GAPDH chr12 6534512 6538374 + EXOSC4 chr8 144078648 144080648 + RPS14 chr5 150442635 150449739 - WDR46 chr6 33279108 33289247 - TCP1 chr6 159778498 159789703 - PITX2 chr4 110617423 110642123 - FNDC3A chr13 48975912 49209779 + PFN1 chr17 4945652 4949061 - FKBP1A chr20 1368978 1393172 - HS6ST1 chr2 128236716 128318868 - DTL chr1 212035553 212107400 + TNIK chr3 171058414 171460408 - GDAP1 chr8 74321130 74488872 + ACOX3 chr4 8366282 8440723 - YME1L1 chr10 27110112 27155266 - ERP29 chr12 112013348 112023449 + MT-CO1 chrMT 5904 7445 + PABPC4 chr1 39560816 39576790 - MT-ND2 chrMT 4470 5511 + BOD1 chr5 173607145 173616659 - NUCKS1 chr1 205712822 205750182 - SPG7 chr16 89490719 89557768 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-193a-3p chr17 31560050 31560071 + hsa-miR-485-5p chr14 101055427 101055448 + hsa-miR-193b-3p chr16 14304017 14304038 + hsa-miR-650 chr22 22822791 22822811 + hsa-miR-423-5p chr17 30117095 30117117 + hsa-miR-664b-5p chrX 154768596 154768619 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC016876.2 chr17 7581964 7584086 - AL355987.4 chr9 136799223 136810042 + SNHG14 chr15 24978583 25420336 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.