exRNAdisease

Entry Detail

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General Information

Database ID: exR0359461 RNA Name: AFF1 RNA Type: mRNA Chromosome: chr4 Starnd: + Coordinate: Start Site(bp): 86935002 End Site(bp): 87141054 External Links: ENSG00000172493

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AP1G1 chr16 71729000 71809201 - TMBIM6 chr12 49707725 49764934 + UBALD2 chr17 76265348 76271298 + KIF1C chr17 4997950 5028401 + ERAL1 chr17 28855010 28861061 + HNRNPM chr19 8444767 8489114 + DUSP5 chr10 110497907 110511533 + VPS13A chr9 77177445 77421537 + RAB5C chr17 42124976 42155044 - RAVER2 chr1 64745095 64833232 + RSU1 chr10 16590611 16817463 - DNAJC5 chr20 63895126 63936031 + RHEB chr7 151466012 151520120 - TSR1 chr17 2322396 2336657 - CHD8 chr14 21385194 21456126 - UNC13D chr17 75827225 75844717 - MCM2 chr3 127598410 127622436 + RANBP2 chr2 108719482 108785809 + ACTG1 chr17 81509971 81523847 - PRDX6 chr1 173477330 173488815 + BAZ1B chr7 73440406 73522293 - ABI1 chr10 26746593 26861087 - KRAS chr12 25205246 25250936 - STRBP chr9 123109500 123268586 - CC2D1A chr19 13906201 13930879 + COPS8 chr2 237085882 237100474 + DEDD chr1 161120974 161132688 - SSRP1 chr11 57325986 57335892 - CASP8 chr2 201233443 201287711 + TMEM237 chr2 201620184 201643570 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC025181.2 chr5 32174471 32175272 + MAPKAPK5-AS1 chr12 111839764 111842902 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.