exRNAdisease

Entry Detail

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General Information

Database ID: exR0359465 RNA Name: AFG3L2 RNA Type: mRNA Chromosome: chr18 Starnd: - Coordinate: Start Site(bp): 12328944 End Site(bp): 12377227 External Links: ENSG00000141385

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand RPS18 chr6 33272075 33276511 + RPS23 chr5 82273320 82278396 - SOX11 chr2 5692384 5701385 + MT-CO2 chrMT 7586 8269 + RPL3 chr22 39312882 39320389 - N4BP2L2 chr13 32432417 32538885 - CHMP5 chr9 33264879 33282070 + PEAK1 chr15 77100656 77420144 - PPP1CB chr2 28751640 28802940 + GDI2 chr10 5765223 5842132 - PITX1 chr5 135027734 135034813 - NUP107 chr12 68686951 68745809 + DYNLL1 chr12 120469850 120498493 + CAPN2 chr1 223701593 223776018 + CSDE1 chr1 114716913 114758676 - ALDOA chr16 30064164 30070457 + SLC41A1 chr1 205789094 205813748 - TRAPPC1 chr17 7930345 7932123 - ADH5 chr4 99070978 99088801 - RPL35 chr9 124857880 124861981 - LCP1 chr13 46125920 46211871 - GAPDH chr12 6534512 6538374 + FLNA chrX 154348524 154374638 - BTG2 chr1 203305491 203309602 + FASN chr17 82078338 82098294 - C17orf49 chr17 7014495 7017525 + HK2 chr2 74834127 74893359 + YBX1 chr1 42682418 42703805 + THOC5 chr22 29505879 29555216 - PTTG1IP chr21 44849585 44873903 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC091564.7 chr11 6610883 6616594 - AP001496.2 chr18 5232876 5238526 - SNHG1 chr11 62851984 62855953 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.