exRNAdisease

Entry Detail

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General Information

Database ID: exR0359468 RNA Name: AFTPH RNA Type: mRNA Chromosome: chr2 Starnd: + Coordinate: Start Site(bp): 64524305 End Site(bp): 64593005 External Links: ENSG00000119844

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand BID chr22 17734138 17774770 - YWHAQ chr2 9583967 9630997 - CLEC2D chr12 9664969 9699553 + NAP1L1 chr12 76036585 76084958 - DTYMK chr2 241675747 241686944 - TWSG1 chr18 9334767 9402420 + TUBA1B chr12 49127782 49131397 - LAMC1 chr1 183023420 183145592 + BIRC2 chr11 102347211 102378670 + CUL3 chr2 224470150 224585397 - PGK1 chrX 77910739 78129295 + HLA-A chr6 29941260 29945884 + HEXA chr15 72340919 72376476 - STRN4 chr19 46719511 46746994 - RPUSD4 chr11 126202096 126211692 - HLA-F chr6 29722775 29738528 + BRPF3 chr6 36196744 36232790 + CD48 chr1 160678746 160711831 - ATP5MG chr11 118401606 118431496 + LCP1 chr13 46125920 46211871 - ATP8B2 chr1 154325553 154351304 + NCL chr2 231453531 231483641 - AKIRIN2 chr6 87674860 87702233 - HNRNPU chr1 244840638 244864560 - HDAC2 chr6 113933028 114011308 - PHF20 chr20 35771974 35950370 + RPL32 chr3 12834485 12841582 - MRPL4 chr19 10251901 10260055 + RPL27 chr17 42998273 43002959 + YWHAH chr22 31944522 31957603 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand LINC01405 chr12 110934590 110959093 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.