exRNAdisease

Entry Detail

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General Information

Database ID: exR0359471 RNA Name: AGAP3 RNA Type: mRNA Chromosome: chr7 Starnd: + Coordinate: Start Site(bp): 151085831 End Site(bp): 151144436 External Links: ENSG00000133612

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ZNF703 chr8 37695782 37700019 + INTS1 chr7 1470277 1504389 - AC010646.1 chr19 17235940 17255448 - RNF11 chr1 51236273 51273447 + SLC31A1 chr9 113221544 113264492 + GFM1 chr3 158644278 158692575 + NR2F6 chr19 17231883 17245940 - DOCK1 chr10 126905409 127452517 + TMEM181 chr6 158536436 158635428 + TMEM241 chr18 23197144 23437961 - CDC25A chr3 48157146 48188417 - POU5F1B chr8 127322183 127420066 + LONP1 chr19 5691834 5720572 - KRT10 chr17 40818117 40822614 - XYLT2 chr17 50346126 50363138 + KDM4B chr19 4969113 5153598 + BSG chr19 571277 583493 + RPS6 chr9 19375715 19380236 - PDGFA chr7 497258 520296 - FAM219B chr15 74899992 74906883 - HOXD11 chr2 176104216 176109754 + ADA chr20 44619522 44652233 - HMGA1 chr6 34236873 34246231 + CDK18 chr1 205504595 205532793 + SLC25A39 chr17 44319625 44324870 - ADIPOR1 chr1 202940826 202958572 - MT-ND4L chrMT 10470 10766 + INA chr10 103277138 103290346 + WDR6 chr3 49007062 49015953 + BUB3 chr10 123154402 123170467 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-299-5p chr14 101023800 101023821 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC022400.3 chr10 73674295 73730466 - AC087741.1 chr17 80200673 80205949 - AC079949.1 chr12 127142029 127146532 - AL355472.2 chr1 234357006 234365828 + AL356488.2 chr1 109100193 109100619 + FGD5-AS1 chr3 14920347 14948424 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.