exRNAdisease

Entry Detail

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General Information

Database ID: exR0359472 RNA Name: AGAP4 RNA Type: mRNA Chromosome: chr10 Starnd: - Coordinate: Start Site(bp): 45825594 End Site(bp): 45853875 External Links: ENSG00000188234

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AGAP1 chr2 235494043 236131800 + AGAP5 chr10 73674285 73698159 - AGAP6 chr10 49982190 50010499 + AGAP9 chr10 47501854 47523638 - BMS1 chr10 42782795 42834937 + BSG chr19 571277 583493 + CCT3 chr1 156308968 156367873 - CDC42 chr1 22025511 22101360 + CNBP chr3 129169484 129183922 - FASN chr17 82078338 82098294 - FZD9 chr7 73433778 73436120 + HYPK chr15 43796142 43803043 + KIAA1522 chr1 32741830 32774970 + MATR3 chr5 139273752 139331671 + NONO chrX 71283192 71301168 + PHF3 chr6 63635820 63779336 + RPL37 chr5 40825262 40835222 - RPS2 chr16 1962058 1964841 - RPS9 chr19 54200809 54249003 + SERF2 chr15 43777087 43802589 + TSR1 chr17 2322396 2336657 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC022400.3 chr10 73674295 73730466 - AGAP11 chr10 86970741 87010126 + AL136982.1 chr10 86992406 87010203 + AC135983.2 chr15 32398956 32435233 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.