exRNAdisease

Entry Detail

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General Information

Database ID: exR0359481 RNA Name: AGFG1 RNA Type: mRNA Chromosome: chr2 Starnd: + Coordinate: Start Site(bp): 227472152 End Site(bp): 227561214 External Links: ENSG00000173744

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand MTRNR2L4 chr16 3370979 3372668 - MRPS7 chr17 75261674 75266376 + PCDH7 chr4 30720415 31146805 + GNAS chr20 58839718 58911192 + COMMD1 chr2 61888724 62147247 + FLOT2 chr17 28879335 28897733 - UBE2W chr8 73780097 73878910 - C6orf47 chr6 31658298 31660778 - TUBA1B chr12 49127782 49131397 - P2RX1 chr17 3896592 3916476 - NDUFS7 chr19 1383527 1395589 + BRD3 chr9 134030305 134068535 - CCDC127 chr5 196868 218153 - LAMP1 chr13 113297239 113323672 + LSP1 chr11 1852970 1892267 + RPSA chr3 39406716 39412542 + PIP4K2C chr12 57591174 57603418 + RACK1 chr5 181236897 181248096 - EZH2 chr7 148807383 148884321 - MEF2A chr15 99565417 99716466 + BSG chr19 571277 583493 + REXO1 chr19 1815248 1848483 - ID1 chr20 31605283 31606515 + MXD4 chr4 2247432 2262109 - RPL27A chr11 8682788 8714759 + FEM1B chr15 68277745 68295862 + RPS23 chr5 82273320 82278396 - AC116366.3 chr5 132410832 132646079 + MRPL10 chr17 47823272 47831541 - ATP5MPL chr14 103912288 103928269 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-106b-5p chr7 100094043 100094063 - hsa-miR-380-3p chr14 101025056 101025077 + hsa-miR-1270 chr19 20399320 20399342 - hsa-miR-4492 chr11 118910765 118910781 + hsa-miR-5703 chr2 227472162 227472179 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AD000090.1 chr19 35557956 35581954 + AP001330.4 chr8 101208148 101208558 + GAS5 chr1 173858559 173868882 - HOXA-AS2 chr7 27107777 27134302 + NEAT1 chr11 65422774 65445540 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.