exRNAdisease

Entry Detail

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General Information

Database ID: exR0359482 RNA Name: AGFG2 RNA Type: mRNA Chromosome: chr7 Starnd: + Coordinate: Start Site(bp): 100539203 End Site(bp): 100568220 External Links: ENSG00000106351

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand HK1 chr10 69269984 69401884 + DUSP2 chr2 96143169 96145440 - HMGA1 chr6 34236873 34246231 + ADCY3 chr2 24819169 24919839 - BLCAP chr20 37492472 37527931 - KCNQ5 chr6 72621792 73198851 + LASP1 chr17 38869859 38921770 + RPS5 chr19 58386400 58394806 + CORO1B chr11 67435510 67443821 - RBM42 chr19 35629030 35637686 + DDX5 chr17 64498254 64508199 - RPS19 chr19 41860255 41872925 + REEP5 chr5 112876385 112922289 - HLA-A chr6 29941260 29945884 + VPS4A chr16 69311350 69326939 + SLC12A8 chr3 125082636 125212864 - ZFP36L1 chr14 68787660 68796253 - HLA-E chr6 30489509 30494194 + ARID3A chr19 925781 975939 + RPL13 chr16 89560657 89566828 + PTPRCAP chr11 67435510 67437682 - SOX13 chr1 204073115 204127743 + NMT1 chr17 45051610 45109016 + CRKL chr22 20917407 20953747 + INAFM2 chr15 40323692 40326715 + CDK1 chr10 60778331 60794852 + TUBA1B chr12 49127782 49131397 - BASP1 chr5 17065598 17276843 + TMEM54 chr1 32894594 32901438 - TPT1 chr13 45333471 45341370 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC009065.3 chr16 2091436 2095433 + TMEM147-AS1 chr19 35540738 35546029 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.