exRNAdisease

Entry Detail

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General Information

Database ID: exR0359483 RNA Name: AGGF1 RNA Type: mRNA Chromosome: chr5 Starnd: + Coordinate: Start Site(bp): 77029251 End Site(bp): 77065234 External Links: ENSG00000164252

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ASPM chr1 197084127 197146694 - RPL27A chr11 8682788 8714759 + PGK1 chrX 77910739 78129295 + TUBA1B chr12 49127782 49131397 - EIF5A chr17 7306999 7312463 + FP565260.6 chr21 5155499 5165472 - MYL6 chr12 56158161 56163496 + FP565260.2 chr21 5116343 5133805 - ESRP1 chr8 94641074 94707466 + SUB1 chr5 32531633 32604079 + BZW2 chr7 16646131 16706523 + ACTA1 chr1 229431245 229434098 - B2M chr15 44711487 44718877 + NEDD4L chr18 58044226 58401540 + HLA-A chr6 29941260 29945884 + MARCKSL1 chr1 32333839 32336233 - RPS9 chr19 54200809 54249003 + AC008581.2 chr5 77030902 77152155 + KIFC3 chr16 57758217 57863053 - SMC4 chr3 160399274 160434954 + PRRC1 chr5 127517609 127555089 + TCEAL4 chrX 103576231 103587736 + PPP2R5B chr11 64917553 64934475 + ERAL1 chr17 28855010 28861061 + PBX1 chr1 164555584 164899296 + ID1 chr20 31605283 31606515 + CCPG1 chr15 55340032 55408510 - RPL23A chr17 28719985 28724359 + ACTN1 chr14 68874143 68979440 - MIF chr22 23894383 23895227 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand FGD5-AS1 chr3 14920347 14948424 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.